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Applied Barcoding: The Practicalities of DNA Testing for Herbals

DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharma...

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Detalles Bibliográficos
Autores principales: Howard, Caroline, Lockie-Williams, Claire, Slater, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570336/
https://www.ncbi.nlm.nih.gov/pubmed/32899738
http://dx.doi.org/10.3390/plants9091150
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author Howard, Caroline
Lockie-Williams, Claire
Slater, Adrian
author_facet Howard, Caroline
Lockie-Williams, Claire
Slater, Adrian
author_sort Howard, Caroline
collection PubMed
description DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.
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spelling pubmed-75703362020-10-28 Applied Barcoding: The Practicalities of DNA Testing for Herbals Howard, Caroline Lockie-Williams, Claire Slater, Adrian Plants (Basel) Article DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals. MDPI 2020-09-04 /pmc/articles/PMC7570336/ /pubmed/32899738 http://dx.doi.org/10.3390/plants9091150 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Howard, Caroline
Lockie-Williams, Claire
Slater, Adrian
Applied Barcoding: The Practicalities of DNA Testing for Herbals
title Applied Barcoding: The Practicalities of DNA Testing for Herbals
title_full Applied Barcoding: The Practicalities of DNA Testing for Herbals
title_fullStr Applied Barcoding: The Practicalities of DNA Testing for Herbals
title_full_unstemmed Applied Barcoding: The Practicalities of DNA Testing for Herbals
title_short Applied Barcoding: The Practicalities of DNA Testing for Herbals
title_sort applied barcoding: the practicalities of dna testing for herbals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570336/
https://www.ncbi.nlm.nih.gov/pubmed/32899738
http://dx.doi.org/10.3390/plants9091150
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