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Single-Cell Elasticity Measurement with an Optically Actuated Microrobot

A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grow...

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Autores principales: Grexa, István, Fekete, Tamás, Molnár, Judit, Molnár, Kinga, Vizsnyiczai, Gaszton, Ormos, Pál, Kelemen, Lóránd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570390/
https://www.ncbi.nlm.nih.gov/pubmed/32972024
http://dx.doi.org/10.3390/mi11090882
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author Grexa, István
Fekete, Tamás
Molnár, Judit
Molnár, Kinga
Vizsnyiczai, Gaszton
Ormos, Pál
Kelemen, Lóránd
author_facet Grexa, István
Fekete, Tamás
Molnár, Judit
Molnár, Kinga
Vizsnyiczai, Gaszton
Ormos, Pál
Kelemen, Lóránd
author_sort Grexa, István
collection PubMed
description A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments.
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spelling pubmed-75703902020-10-28 Single-Cell Elasticity Measurement with an Optically Actuated Microrobot Grexa, István Fekete, Tamás Molnár, Judit Molnár, Kinga Vizsnyiczai, Gaszton Ormos, Pál Kelemen, Lóránd Micromachines (Basel) Article A cell elasticity measurement method is introduced that uses polymer microtools actuated by holographic optical tweezers. The microtools were prepared with two-photon polymerization. Their shape enables the approach of the cells in any lateral direction. In the presented case, endothelial cells grown on vertical polymer walls were probed by the tools in a lateral direction. The use of specially shaped microtools prevents the target cells from photodamage that may arise during optical trapping. The position of the tools was recorded simply with video microscopy and analyzed with image processing methods. We critically compare the resulting Young’s modulus values to those in the literature obtained by other methods. The application of optical tweezers extends the force range available for cell indentations measurements down to the fN regime. Our approach demonstrates a feasible alternative to the usual vertical indentation experiments. MDPI 2020-09-22 /pmc/articles/PMC7570390/ /pubmed/32972024 http://dx.doi.org/10.3390/mi11090882 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grexa, István
Fekete, Tamás
Molnár, Judit
Molnár, Kinga
Vizsnyiczai, Gaszton
Ormos, Pál
Kelemen, Lóránd
Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title_full Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title_fullStr Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title_full_unstemmed Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title_short Single-Cell Elasticity Measurement with an Optically Actuated Microrobot
title_sort single-cell elasticity measurement with an optically actuated microrobot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570390/
https://www.ncbi.nlm.nih.gov/pubmed/32972024
http://dx.doi.org/10.3390/mi11090882
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