Conformation of G-quadruplex Controlled by Click Reaction

G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformatio...

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Autores principales: Xiao, Chao-Da, He, Zhi-Yong, Guo, Chuan-Xin, Shen, Xiang-Chun, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570587/
https://www.ncbi.nlm.nih.gov/pubmed/32971833
http://dx.doi.org/10.3390/molecules25184339
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author Xiao, Chao-Da
He, Zhi-Yong
Guo, Chuan-Xin
Shen, Xiang-Chun
Xu, Yan
author_facet Xiao, Chao-Da
He, Zhi-Yong
Guo, Chuan-Xin
Shen, Xiang-Chun
Xu, Yan
author_sort Xiao, Chao-Da
collection PubMed
description G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2′-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence.
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spelling pubmed-75705872020-10-28 Conformation of G-quadruplex Controlled by Click Reaction Xiao, Chao-Da He, Zhi-Yong Guo, Chuan-Xin Shen, Xiang-Chun Xu, Yan Molecules Article G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2′-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence. MDPI 2020-09-22 /pmc/articles/PMC7570587/ /pubmed/32971833 http://dx.doi.org/10.3390/molecules25184339 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Chao-Da
He, Zhi-Yong
Guo, Chuan-Xin
Shen, Xiang-Chun
Xu, Yan
Conformation of G-quadruplex Controlled by Click Reaction
title Conformation of G-quadruplex Controlled by Click Reaction
title_full Conformation of G-quadruplex Controlled by Click Reaction
title_fullStr Conformation of G-quadruplex Controlled by Click Reaction
title_full_unstemmed Conformation of G-quadruplex Controlled by Click Reaction
title_short Conformation of G-quadruplex Controlled by Click Reaction
title_sort conformation of g-quadruplex controlled by click reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570587/
https://www.ncbi.nlm.nih.gov/pubmed/32971833
http://dx.doi.org/10.3390/molecules25184339
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AT xuyan conformationofgquadruplexcontrolledbyclickreaction

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