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Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species

The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insu...

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Autores principales: Etsassala, Ninon G. E. R., Ndjoubi, Kadidiatou O., Mbira, Thilly J., Pearce, Brendon, Pearce, Keenau, Iwuoha, Emmanuel I., Hussein, Ahmed A., Benjeddou, Mongi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570927/
https://www.ncbi.nlm.nih.gov/pubmed/32927596
http://dx.doi.org/10.3390/molecules25184129
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author Etsassala, Ninon G. E. R.
Ndjoubi, Kadidiatou O.
Mbira, Thilly J.
Pearce, Brendon
Pearce, Keenau
Iwuoha, Emmanuel I.
Hussein, Ahmed A.
Benjeddou, Mongi
author_facet Etsassala, Ninon G. E. R.
Ndjoubi, Kadidiatou O.
Mbira, Thilly J.
Pearce, Brendon
Pearce, Keenau
Iwuoha, Emmanuel I.
Hussein, Ahmed A.
Benjeddou, Mongi
author_sort Etsassala, Ninon G. E. R.
collection PubMed
description The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic β-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely, Salvia africana-lutea, Leonotis ocymifolia, and Plectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds 1 (p = 0.0031), 8 (p = 0.0053), and 6 (p = 0.0086) showed a marked increase in glucose uptake, respectively. Additionally, 1, 4, and 6 exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of ~70%, ~68%, and ~67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes.
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spelling pubmed-75709272020-10-28 Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species Etsassala, Ninon G. E. R. Ndjoubi, Kadidiatou O. Mbira, Thilly J. Pearce, Brendon Pearce, Keenau Iwuoha, Emmanuel I. Hussein, Ahmed A. Benjeddou, Mongi Molecules Article The prevalence of diabetes mellitus (DM), considered one of the most common metabolic disorders, has dramatically increased and resulted in higher rates of morbidity and mortality around the world in the past decade. It is well known that insulin resistance in target tissues and a deficiency in insulin secretion from pancreatic β-cells are the main characteristics of type 2 diabetes. The aim of this study was the bio-evaluation of compounds isolated from three selected plant species: namely, Salvia africana-lutea, Leonotis ocymifolia, and Plectranthus madagascariensis, for their glucose-uptake ability. Methanolic extracts were produced from the aerial parts of each plant. Compounds were identified using different spectroscopic techniques. The glucose-uptake ability of each compound was then evaluated in mammalian cells using 2-deoxyglucose-6-phosphate. The cytotoxicity of each compound was established via the MTT assay. Chromatographic purification of the three plant species yielded sixteen pure terpenoids. Compounds 1 (p = 0.0031), 8 (p = 0.0053), and 6 (p = 0.0086) showed a marked increase in glucose uptake, respectively. Additionally, 1, 4, and 6 exhibited cytotoxicity toward mammalian tissue with a decrease in cell viability of ~70%, ~68%, and ~67%, respectively. The results suggested that several compounds demonstrated a marked increase in glucose uptake, while two of the compounds exhibited signs of cytotoxicity. It may, therefore, be suggested that these compounds be considered as potential candidates for novel plant-derived alternative therapies in the treatment of type 2 diabetes. MDPI 2020-09-10 /pmc/articles/PMC7570927/ /pubmed/32927596 http://dx.doi.org/10.3390/molecules25184129 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Etsassala, Ninon G. E. R.
Ndjoubi, Kadidiatou O.
Mbira, Thilly J.
Pearce, Brendon
Pearce, Keenau
Iwuoha, Emmanuel I.
Hussein, Ahmed A.
Benjeddou, Mongi
Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title_full Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title_fullStr Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title_full_unstemmed Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title_short Glucose-Uptake Activity and Cytotoxicity of Diterpenes and Triterpenes Isolated from Lamiaceae Plant Species
title_sort glucose-uptake activity and cytotoxicity of diterpenes and triterpenes isolated from lamiaceae plant species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570927/
https://www.ncbi.nlm.nih.gov/pubmed/32927596
http://dx.doi.org/10.3390/molecules25184129
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