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The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer

(1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lu...

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Autores principales: Yang, Hui, Heyer, Jasmine, Zhao, Hui, Liang, Shengxian, Guo, Rui, Zhong, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571067/
https://www.ncbi.nlm.nih.gov/pubmed/32927648
http://dx.doi.org/10.3390/molecules25184136
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author Yang, Hui
Heyer, Jasmine
Zhao, Hui
Liang, Shengxian
Guo, Rui
Zhong, Li
author_facet Yang, Hui
Heyer, Jasmine
Zhao, Hui
Liang, Shengxian
Guo, Rui
Zhong, Li
author_sort Yang, Hui
collection PubMed
description (1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lung fibroblast MRC-5 cells using Western blot and immunofluorescence assay. Cathepsin K was transiently overexpressed or knocked down using transfection with a recombinant plasmid and siRNA, respectively, to test the effects on cell proliferation, migration, invasion, and on the mammalian target of rapamycin (mTOR) signaling pathway. (3) Results: Expression of Cathepsin K was increased significantly in A549 cells and diffused within the cytoplasm compared to the MRC-5 cells used as control. Cathepsin K overexpression promoted the proliferation, migration, and invasion of A549 cells, accompanied by mTOR activation. Cathepsin K knockdown reversed the above malignant behavior and inhibited the mTOR signaling activation, suggesting that Cathepsin K may promote the progression of NSCLC by activating the mTOR signaling pathway. (4) Conclusion: Cathepsin K may potentially represent a viable drug target for NSCLC treatment.
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spelling pubmed-75710672020-10-28 The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer Yang, Hui Heyer, Jasmine Zhao, Hui Liang, Shengxian Guo, Rui Zhong, Li Molecules Article (1) Background: Cathepsin K has been found overexpressed in several malignant tumors. However, there is little information regarding the involvement of Cathepsin K in non-small cell lung cancer (NSCLC). (2) Methods: Cathepsin K expression was tested in human NSCLC cell lines A549 and human embryo lung fibroblast MRC-5 cells using Western blot and immunofluorescence assay. Cathepsin K was transiently overexpressed or knocked down using transfection with a recombinant plasmid and siRNA, respectively, to test the effects on cell proliferation, migration, invasion, and on the mammalian target of rapamycin (mTOR) signaling pathway. (3) Results: Expression of Cathepsin K was increased significantly in A549 cells and diffused within the cytoplasm compared to the MRC-5 cells used as control. Cathepsin K overexpression promoted the proliferation, migration, and invasion of A549 cells, accompanied by mTOR activation. Cathepsin K knockdown reversed the above malignant behavior and inhibited the mTOR signaling activation, suggesting that Cathepsin K may promote the progression of NSCLC by activating the mTOR signaling pathway. (4) Conclusion: Cathepsin K may potentially represent a viable drug target for NSCLC treatment. MDPI 2020-09-10 /pmc/articles/PMC7571067/ /pubmed/32927648 http://dx.doi.org/10.3390/molecules25184136 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Hui
Heyer, Jasmine
Zhao, Hui
Liang, Shengxian
Guo, Rui
Zhong, Li
The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title_full The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title_fullStr The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title_full_unstemmed The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title_short The Potential Role of Cathepsin K in Non-Small Cell Lung Cancer
title_sort potential role of cathepsin k in non-small cell lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571067/
https://www.ncbi.nlm.nih.gov/pubmed/32927648
http://dx.doi.org/10.3390/molecules25184136
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