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A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate

A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is th...

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Autores principales: Tyszczuk-Rotko, Katarzyna, Kozak, Jędrzej, Sztanke, Małgorzata, Sztanke, Krzysztof, Sadok, Ilona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571158/
https://www.ncbi.nlm.nih.gov/pubmed/32933116
http://dx.doi.org/10.3390/s20185217
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author Tyszczuk-Rotko, Katarzyna
Kozak, Jędrzej
Sztanke, Małgorzata
Sztanke, Krzysztof
Sadok, Ilona
author_facet Tyszczuk-Rotko, Katarzyna
Kozak, Jędrzej
Sztanke, Małgorzata
Sztanke, Krzysztof
Sadok, Ilona
author_sort Tyszczuk-Rotko, Katarzyna
collection PubMed
description A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is the first method for the quantitative determination of EIMTC. The modified screen-printed sensor exhibited excellent electrochemical activity in reducing EIMTC. The peak current of EIMTC was found to be linear in two concentration ranges of 2.0 × 10(−9) – 2.0 × 10(−8) mol L(−1) and 2.0 × 10(−8) – 2.0 × 10(−7) mol L(−1), with a detection limit of 5.0 × 10(−10) mol L(−1). The connection of flow-cell for the SPCE/CNFs with SWV detection allowed for the successful determination of EIMTC in human serum samples. Ultra-high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS) acted as a comparative method in the serum samples analysis.
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spelling pubmed-75711582020-10-28 A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate Tyszczuk-Rotko, Katarzyna Kozak, Jędrzej Sztanke, Małgorzata Sztanke, Krzysztof Sadok, Ilona Sensors (Basel) Article A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-c][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is the first method for the quantitative determination of EIMTC. The modified screen-printed sensor exhibited excellent electrochemical activity in reducing EIMTC. The peak current of EIMTC was found to be linear in two concentration ranges of 2.0 × 10(−9) – 2.0 × 10(−8) mol L(−1) and 2.0 × 10(−8) – 2.0 × 10(−7) mol L(−1), with a detection limit of 5.0 × 10(−10) mol L(−1). The connection of flow-cell for the SPCE/CNFs with SWV detection allowed for the successful determination of EIMTC in human serum samples. Ultra-high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS) acted as a comparative method in the serum samples analysis. MDPI 2020-09-13 /pmc/articles/PMC7571158/ /pubmed/32933116 http://dx.doi.org/10.3390/s20185217 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tyszczuk-Rotko, Katarzyna
Kozak, Jędrzej
Sztanke, Małgorzata
Sztanke, Krzysztof
Sadok, Ilona
A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title_full A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title_fullStr A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title_full_unstemmed A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title_short A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
title_sort screen-printed sensor coupled with flow system for quantitative determination of a novel promising anticancer agent candidate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571158/
https://www.ncbi.nlm.nih.gov/pubmed/32933116
http://dx.doi.org/10.3390/s20185217
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