The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division

The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within...

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Autores principales: Otsuki, Junko, Iwasaki, Toshiroh, Enatsu, Noritoshi, Katada, Yuya, Furuhashi, Kohyu, Shiotani, Masahide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571684/
https://www.ncbi.nlm.nih.gov/pubmed/33075059
http://dx.doi.org/10.1371/journal.pone.0240936
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author Otsuki, Junko
Iwasaki, Toshiroh
Enatsu, Noritoshi
Katada, Yuya
Furuhashi, Kohyu
Shiotani, Masahide
author_facet Otsuki, Junko
Iwasaki, Toshiroh
Enatsu, Noritoshi
Katada, Yuya
Furuhashi, Kohyu
Shiotani, Masahide
author_sort Otsuki, Junko
collection PubMed
description The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within blastomeres as cellular markers. Our study found that the cellular composition of the ICM in humans is largely determined at the time of the fourth division and blastomeres which cleave first to fourth, during the fourth division from 8 cells to 16 cells, have the potential to be incorporated in the ICM.
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spelling pubmed-75716842020-10-26 The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division Otsuki, Junko Iwasaki, Toshiroh Enatsu, Noritoshi Katada, Yuya Furuhashi, Kohyu Shiotani, Masahide PLoS One Research Article The fate of the ICM in humans is still unknown, due to the ethical difficulties surrounding experimentation in this field. In this study we have explored the existing time-lapse recording data of embryos in the early stages of development, taking advantage of the large refractile bodies (RBs) within blastomeres as cellular markers. Our study found that the cellular composition of the ICM in humans is largely determined at the time of the fourth division and blastomeres which cleave first to fourth, during the fourth division from 8 cells to 16 cells, have the potential to be incorporated in the ICM. Public Library of Science 2020-10-19 /pmc/articles/PMC7571684/ /pubmed/33075059 http://dx.doi.org/10.1371/journal.pone.0240936 Text en © 2020 Otsuki et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Otsuki, Junko
Iwasaki, Toshiroh
Enatsu, Noritoshi
Katada, Yuya
Furuhashi, Kohyu
Shiotani, Masahide
The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title_full The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title_fullStr The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title_full_unstemmed The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title_short The inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
title_sort inclusion of blastomeres into the inner cell mass in early-stage human embryos depends on the sequence of cell cleavages during the fourth division
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7571684/
https://www.ncbi.nlm.nih.gov/pubmed/33075059
http://dx.doi.org/10.1371/journal.pone.0240936
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