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Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572389/ https://www.ncbi.nlm.nih.gov/pubmed/33077772 http://dx.doi.org/10.1038/s41598-020-74735-0 |
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author | Yamano-Adachi, Noriko Arishima, Rintaro Puriwat, Sukwattananipaat Omasa, Takeshi |
author_facet | Yamano-Adachi, Noriko Arishima, Rintaro Puriwat, Sukwattananipaat Omasa, Takeshi |
author_sort | Yamano-Adachi, Noriko |
collection | PubMed |
description | Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate much faster than conventional CHO cells and general mammalian cells. Transgenes could be introduced into CHL-YN cells with high efficiency. Finally, between 50% to > 100% of the amount of glycosylated immunoglobulin G (IgG)1 produced by CHO-K1 cells was produced by CHL-YN cells over a shorter period of time. In summary, fast-growing CHL-YN cells are a unique cell line for producing recombinant proteins. |
format | Online Article Text |
id | pubmed-7572389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-75723892020-10-21 Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production Yamano-Adachi, Noriko Arishima, Rintaro Puriwat, Sukwattananipaat Omasa, Takeshi Sci Rep Article Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. They are also valuable for their ability to grow in serum-free suspension cultures. In this study, we established a cell line derived from lung tissue of Chinese hamsters, named Chinese hamster lung (CHL)-YN cells. The biosafety of CHL-YN cells was confirmed by in vitro sterility testing, mycoplasma detection, and reverse transcriptase assays. One of the key characteristics of CHL-YN cells was their doubling time of 8.1 h in chemically defined culture medium; thus, they proliferate much faster than conventional CHO cells and general mammalian cells. Transgenes could be introduced into CHL-YN cells with high efficiency. Finally, between 50% to > 100% of the amount of glycosylated immunoglobulin G (IgG)1 produced by CHO-K1 cells was produced by CHL-YN cells over a shorter period of time. In summary, fast-growing CHL-YN cells are a unique cell line for producing recombinant proteins. Nature Publishing Group UK 2020-10-19 /pmc/articles/PMC7572389/ /pubmed/33077772 http://dx.doi.org/10.1038/s41598-020-74735-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yamano-Adachi, Noriko Arishima, Rintaro Puriwat, Sukwattananipaat Omasa, Takeshi Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title | Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title_full | Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title_fullStr | Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title_full_unstemmed | Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title_short | Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production |
title_sort | establishment of fast-growing serum-free immortalised cells from chinese hamster lung tissues for biopharmaceutical production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572389/ https://www.ncbi.nlm.nih.gov/pubmed/33077772 http://dx.doi.org/10.1038/s41598-020-74735-0 |
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