Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes

The utility of COI DNA barcodes in species delimitation is explored as well as life stage associations of five closely related Propsilocerus species: Propsilocerus akamusi (Tokunaga, 1938), Propsilocerus paradoxus (Lundström, 1915), Propsilocerus saetheri Wang, Liu et Paasivirta, 2007, Propsilocerus...

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Autores principales: Yu, Hai-Jun, Lin, Xiao-Long, Zhang, Rui-Lei, Wang, Qian, Wang, Xin-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572518/
https://www.ncbi.nlm.nih.gov/pubmed/33117065
http://dx.doi.org/10.3897/zookeys.957.54668
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author Yu, Hai-Jun
Lin, Xiao-Long
Zhang, Rui-Lei
Wang, Qian
Wang, Xin-Hua
author_facet Yu, Hai-Jun
Lin, Xiao-Long
Zhang, Rui-Lei
Wang, Qian
Wang, Xin-Hua
author_sort Yu, Hai-Jun
collection PubMed
description The utility of COI DNA barcodes in species delimitation is explored as well as life stage associations of five closely related Propsilocerus species: Propsilocerus akamusi (Tokunaga, 1938), Propsilocerus paradoxus (Lundström, 1915), Propsilocerus saetheri Wang, Liu et Paasivirta, 2007, Propsilocerus sinicus Sæther et Wang, 1996, and Propsilocerus taihuensis (Wen, Zhou et Rong, 1994). Results revealed distinctly larger interspecific than intraspecific divergences and indicated a clear “barcode gap”. In total, 42 COI barcode sequences including 16 newly generated DNA barcodes were applied to seven Barcode Index Numbers (BINs). A neighbor-joining (NJ) tree comprises five well-separated clusters representing five morphospecies. Comments on how to distinguish the larvae of P. akamusi and P. taihuensis are provided.
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spelling pubmed-75725182020-10-27 Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes Yu, Hai-Jun Lin, Xiao-Long Zhang, Rui-Lei Wang, Qian Wang, Xin-Hua Zookeys Research Article The utility of COI DNA barcodes in species delimitation is explored as well as life stage associations of five closely related Propsilocerus species: Propsilocerus akamusi (Tokunaga, 1938), Propsilocerus paradoxus (Lundström, 1915), Propsilocerus saetheri Wang, Liu et Paasivirta, 2007, Propsilocerus sinicus Sæther et Wang, 1996, and Propsilocerus taihuensis (Wen, Zhou et Rong, 1994). Results revealed distinctly larger interspecific than intraspecific divergences and indicated a clear “barcode gap”. In total, 42 COI barcode sequences including 16 newly generated DNA barcodes were applied to seven Barcode Index Numbers (BINs). A neighbor-joining (NJ) tree comprises five well-separated clusters representing five morphospecies. Comments on how to distinguish the larvae of P. akamusi and P. taihuensis are provided. Pensoft Publishers 2020-10-12 /pmc/articles/PMC7572518/ /pubmed/33117065 http://dx.doi.org/10.3897/zookeys.957.54668 Text en Hai-Jun Yu, Xiao-Long Lin, Rui-Lei Zhang, Qian Wang, Xin-Hua Wang http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yu, Hai-Jun
Lin, Xiao-Long
Zhang, Rui-Lei
Wang, Qian
Wang, Xin-Hua
Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title_full Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title_fullStr Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title_full_unstemmed Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title_short Species delimitation and life stage association of Propsilocerus Kieffer, 1923 (Diptera, Chironomidae) using DNA barcodes
title_sort species delimitation and life stage association of propsilocerus kieffer, 1923 (diptera, chironomidae) using dna barcodes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572518/
https://www.ncbi.nlm.nih.gov/pubmed/33117065
http://dx.doi.org/10.3897/zookeys.957.54668
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