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Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics

Invertebrates such as Drosophila melanogaster have proven to be a valuable model organism for studies of the nervous system. In order to control neuronal activity, optogenetics has evolved as a powerful technique enabling non-invasive stimulation using light. This requires light sources that can del...

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Autores principales: Meloni, Ilenia, Sachidanandan, Divya, Thum, Andreas S., Kittel, Robert J., Murawski, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572528/
https://www.ncbi.nlm.nih.gov/pubmed/33077824
http://dx.doi.org/10.1038/s41598-020-74448-4
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author Meloni, Ilenia
Sachidanandan, Divya
Thum, Andreas S.
Kittel, Robert J.
Murawski, Caroline
author_facet Meloni, Ilenia
Sachidanandan, Divya
Thum, Andreas S.
Kittel, Robert J.
Murawski, Caroline
author_sort Meloni, Ilenia
collection PubMed
description Invertebrates such as Drosophila melanogaster have proven to be a valuable model organism for studies of the nervous system. In order to control neuronal activity, optogenetics has evolved as a powerful technique enabling non-invasive stimulation using light. This requires light sources that can deliver patterns of light with high temporal and spatial precision. Currently employed light sources for stimulation of small invertebrates, however, are either limited in spatial resolution or require sophisticated and bulky equipment. In this work, we used smartphone displays for optogenetic control of Drosophila melanogaster. We developed an open-source smartphone app that allows time-dependent display of light patterns and used this to activate and inhibit different neuronal populations in both larvae and adult flies. Characteristic behavioural responses were observed depending on the displayed colour and brightness and in agreement with the activation spectra and light sensitivity of the used channelrhodopsins. By displaying patterns of light, we constrained larval movement and were able to guide larvae on the display. Our method serves as a low-cost high-resolution testbench for optogenetic experiments using small invertebrate species and is particularly appealing to application in neuroscience teaching labs.
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spelling pubmed-75725282020-10-21 Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics Meloni, Ilenia Sachidanandan, Divya Thum, Andreas S. Kittel, Robert J. Murawski, Caroline Sci Rep Article Invertebrates such as Drosophila melanogaster have proven to be a valuable model organism for studies of the nervous system. In order to control neuronal activity, optogenetics has evolved as a powerful technique enabling non-invasive stimulation using light. This requires light sources that can deliver patterns of light with high temporal and spatial precision. Currently employed light sources for stimulation of small invertebrates, however, are either limited in spatial resolution or require sophisticated and bulky equipment. In this work, we used smartphone displays for optogenetic control of Drosophila melanogaster. We developed an open-source smartphone app that allows time-dependent display of light patterns and used this to activate and inhibit different neuronal populations in both larvae and adult flies. Characteristic behavioural responses were observed depending on the displayed colour and brightness and in agreement with the activation spectra and light sensitivity of the used channelrhodopsins. By displaying patterns of light, we constrained larval movement and were able to guide larvae on the display. Our method serves as a low-cost high-resolution testbench for optogenetic experiments using small invertebrate species and is particularly appealing to application in neuroscience teaching labs. Nature Publishing Group UK 2020-10-19 /pmc/articles/PMC7572528/ /pubmed/33077824 http://dx.doi.org/10.1038/s41598-020-74448-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Meloni, Ilenia
Sachidanandan, Divya
Thum, Andreas S.
Kittel, Robert J.
Murawski, Caroline
Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title_full Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title_fullStr Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title_full_unstemmed Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title_short Controlling the behaviour of Drosophila melanogaster via smartphone optogenetics
title_sort controlling the behaviour of drosophila melanogaster via smartphone optogenetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7572528/
https://www.ncbi.nlm.nih.gov/pubmed/33077824
http://dx.doi.org/10.1038/s41598-020-74448-4
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