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Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease
OBJECTIVE: Diabetic kidney disease (DKD) leads to low high albuminuria and gradually progresses to very high albuminuria with kidney insufficiency. However, about 20–40% of DKD is normoalbuminuric DKD (NADKD), which has impaired kidney function but normal urine albumin. This study is to investigate...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573362/ https://www.ncbi.nlm.nih.gov/pubmed/33123032 http://dx.doi.org/10.3389/fphys.2020.578799 |
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author | Feng, Qian Li, Yuanmeng Yang, Yuwei Feng, Jiafu |
author_facet | Feng, Qian Li, Yuanmeng Yang, Yuwei Feng, Jiafu |
author_sort | Feng, Qian |
collection | PubMed |
description | OBJECTIVE: Diabetic kidney disease (DKD) leads to low high albuminuria and gradually progresses to very high albuminuria with kidney insufficiency. However, about 20–40% of DKD is normoalbuminuric DKD (NADKD), which has impaired kidney function but normal urine albumin. This study is to investigate the urine metabolomic profiles of patients with NADKD and albuminuria DKD (ADKD). METHODS: In total, 95 patients were divided into a simple diabetes mellitus group (SDM group), an ADKD group, and a NADKD group. All subjects were analyzed for urine metabolites using non-targeted metabolomics based on ultra-performance liquid chromatography – tandem mass spectrometry. RESULTS: The urine metabolomic profiles of the SDM group, NADKD group, and ADKD group were significantly different, and 65 different metabolites were identified among the three groups. Metabolic pathway analysis of these differential metabolites found that the top three significantly changed metabolic pathways were linoleic acid metabolism, citrate cycle, and, arginine and proline metabolism. There are 12 metabolites enriched in these three metabolic pathways. In detail, compared with those in the SDM group, the levels of γ-linolenic acid in the ADKD group were increased significantly, while the levels of succinic acid, cis-aconitic acid, citric acid, L-proline, L-erythro-4-hydroxyglutamate, N-methylhydantoin, N-carbamoylputrescine, spermidine, and 5-aminopentanoic acid were reduced significantly; compared with those in the NADKD group, the levels of linoleic acid, γ-linolenic acid, and L-malic acid in the ADKD group were increased significantly (P < 0.05), while the levels of L-proline, L-erythro-4-hydroxyglutamate, N-carbamoylputrescine, and spermidine were significantly reduced (P < 0.05). However, there were no significant difference between the SDM group and NADKD group (P > 0.05). CONCLUSION: The urine metabolomic profiles between the NADKD group and the ADKD group are significantly different. Specifically, these two groups have distinct levels of linoleic acid, γ-linolenic acid, L-malic acid, L-proline, L-erythro-4-hydroxyglutamate, N-carbamoylputrescine, and spermidine. |
format | Online Article Text |
id | pubmed-7573362 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75733622020-10-28 Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease Feng, Qian Li, Yuanmeng Yang, Yuwei Feng, Jiafu Front Physiol Physiology OBJECTIVE: Diabetic kidney disease (DKD) leads to low high albuminuria and gradually progresses to very high albuminuria with kidney insufficiency. However, about 20–40% of DKD is normoalbuminuric DKD (NADKD), which has impaired kidney function but normal urine albumin. This study is to investigate the urine metabolomic profiles of patients with NADKD and albuminuria DKD (ADKD). METHODS: In total, 95 patients were divided into a simple diabetes mellitus group (SDM group), an ADKD group, and a NADKD group. All subjects were analyzed for urine metabolites using non-targeted metabolomics based on ultra-performance liquid chromatography – tandem mass spectrometry. RESULTS: The urine metabolomic profiles of the SDM group, NADKD group, and ADKD group were significantly different, and 65 different metabolites were identified among the three groups. Metabolic pathway analysis of these differential metabolites found that the top three significantly changed metabolic pathways were linoleic acid metabolism, citrate cycle, and, arginine and proline metabolism. There are 12 metabolites enriched in these three metabolic pathways. In detail, compared with those in the SDM group, the levels of γ-linolenic acid in the ADKD group were increased significantly, while the levels of succinic acid, cis-aconitic acid, citric acid, L-proline, L-erythro-4-hydroxyglutamate, N-methylhydantoin, N-carbamoylputrescine, spermidine, and 5-aminopentanoic acid were reduced significantly; compared with those in the NADKD group, the levels of linoleic acid, γ-linolenic acid, and L-malic acid in the ADKD group were increased significantly (P < 0.05), while the levels of L-proline, L-erythro-4-hydroxyglutamate, N-carbamoylputrescine, and spermidine were significantly reduced (P < 0.05). However, there were no significant difference between the SDM group and NADKD group (P > 0.05). CONCLUSION: The urine metabolomic profiles between the NADKD group and the ADKD group are significantly different. Specifically, these two groups have distinct levels of linoleic acid, γ-linolenic acid, L-malic acid, L-proline, L-erythro-4-hydroxyglutamate, N-carbamoylputrescine, and spermidine. Frontiers Media S.A. 2020-10-06 /pmc/articles/PMC7573362/ /pubmed/33123032 http://dx.doi.org/10.3389/fphys.2020.578799 Text en Copyright © 2020 Feng, Li, Yang and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Feng, Qian Li, Yuanmeng Yang, Yuwei Feng, Jiafu Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title | Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title_full | Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title_fullStr | Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title_full_unstemmed | Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title_short | Urine Metabolomics Analysis in Patients With Normoalbuminuric Diabetic Kidney Disease |
title_sort | urine metabolomics analysis in patients with normoalbuminuric diabetic kidney disease |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573362/ https://www.ncbi.nlm.nih.gov/pubmed/33123032 http://dx.doi.org/10.3389/fphys.2020.578799 |
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