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Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line
OBJECTIVES: The study was to investigate anti-migration and invasion effects of astaxanthin (ATX), a natural carotenoid derivative distributed in marine environments, against A172 human glioblastoma cells. MATERIALS AND METHODS: Cell viability after ATX treatment was measured by MTT assays. Tumor ce...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573402/ https://www.ncbi.nlm.nih.gov/pubmed/32711429 http://dx.doi.org/10.31557/APJCP.2020.21.7.2029 |
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author | Siangcham, Tanapan Vivithanaporn, Pornpun Sangpairoj, Kant |
author_facet | Siangcham, Tanapan Vivithanaporn, Pornpun Sangpairoj, Kant |
author_sort | Siangcham, Tanapan |
collection | PubMed |
description | OBJECTIVES: The study was to investigate anti-migration and invasion effects of astaxanthin (ATX), a natural carotenoid derivative distributed in marine environments, against A172 human glioblastoma cells. MATERIALS AND METHODS: Cell viability after ATX treatment was measured by MTT assays. Tumor cell migration and invasion were observed by scratch and Boyden chamber assays, respectively. Expression of MMP-2 and activity of MMP-9 were observed by immunoblotting and gelatin zymography, respectively. RESULTS: ATX up to 150 µM was not toxic to A172 cells at 48 h post-treatment. In contrast, ATX at 50 and 100 µM significantly decreased migration and invasion of A172 cells at 24 and 48 h post-treatment. Metastatic-reducing effect of ATX is associated with the reduction of MMP-2 and MMP-9 expressions in a dose-dependent manner. Conclusion: This finding indicated that ATX has anti-migration and invasion effects against human glioblastoma cells and might be applicable for the protection against metastasis of glioblastoma. |
format | Online Article Text |
id | pubmed-7573402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-75734022020-10-30 Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line Siangcham, Tanapan Vivithanaporn, Pornpun Sangpairoj, Kant Asian Pac J Cancer Prev Research Article OBJECTIVES: The study was to investigate anti-migration and invasion effects of astaxanthin (ATX), a natural carotenoid derivative distributed in marine environments, against A172 human glioblastoma cells. MATERIALS AND METHODS: Cell viability after ATX treatment was measured by MTT assays. Tumor cell migration and invasion were observed by scratch and Boyden chamber assays, respectively. Expression of MMP-2 and activity of MMP-9 were observed by immunoblotting and gelatin zymography, respectively. RESULTS: ATX up to 150 µM was not toxic to A172 cells at 48 h post-treatment. In contrast, ATX at 50 and 100 µM significantly decreased migration and invasion of A172 cells at 24 and 48 h post-treatment. Metastatic-reducing effect of ATX is associated with the reduction of MMP-2 and MMP-9 expressions in a dose-dependent manner. Conclusion: This finding indicated that ATX has anti-migration and invasion effects against human glioblastoma cells and might be applicable for the protection against metastasis of glioblastoma. West Asia Organization for Cancer Prevention 2020-07 /pmc/articles/PMC7573402/ /pubmed/32711429 http://dx.doi.org/10.31557/APJCP.2020.21.7.2029 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Siangcham, Tanapan Vivithanaporn, Pornpun Sangpairoj, Kant Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title | Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title_full | Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title_fullStr | Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title_full_unstemmed | Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title_short | Anti-Migration and Invasion Effects of Astaxanthin against A172 Human Glioblastoma Cell Line |
title_sort | anti-migration and invasion effects of astaxanthin against a172 human glioblastoma cell line |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573402/ https://www.ncbi.nlm.nih.gov/pubmed/32711429 http://dx.doi.org/10.31557/APJCP.2020.21.7.2029 |
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