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Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines

OBJECTIVE: In this study, we aimed to harness some solvent extracts of one wild mushroom Hexagonia glabra and test their anti-cancer activity against cervical human cell lines, namelyHeLa, SiHa, and CaSki. METHODS: It includes cell morphological study by microscope, nuclear morphology by DAPI staini...

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Autores principales: Ghosh, Swapan Kumar, Sanyal, Tapojyoti, Bera, Tanmay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573405/
https://www.ncbi.nlm.nih.gov/pubmed/32711423
http://dx.doi.org/10.31557/APJCP.2020.21.7.1977
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author Ghosh, Swapan Kumar
Sanyal, Tapojyoti
Bera, Tanmay
author_facet Ghosh, Swapan Kumar
Sanyal, Tapojyoti
Bera, Tanmay
author_sort Ghosh, Swapan Kumar
collection PubMed
description OBJECTIVE: In this study, we aimed to harness some solvent extracts of one wild mushroom Hexagonia glabra and test their anti-cancer activity against cervical human cell lines, namelyHeLa, SiHa, and CaSki. METHODS: It includes cell morphological study by microscope, nuclear morphology by DAPI staining under fluorescence microscopy, apoptosis assay by fluorescence technique, anti-proliferation by MTT assay and expression of apoptotic and anti-apoptotic genes by Western blotting and cell cycle analysis was done. RESULTS: The selected cervical cancer cells were treated separately with 150 µg/mL of three extracts, namely of ethanolic (EE), ethyl acetate (EAE), and water extract (WE) and exhibited features like round, shrink and dead. All extracts caused apoptosis in cell lines and EE had the highest effect in this regard. The percentages of apoptotic cells in HeLa, SiHa and CaSki, at the same concentration of EE were 79.23, 75.42, and 76.36% respectively. Cytotoxicity assay showed that all three extracts (50 – 250 μg/mL) were potent for inhibition of cell growth of three cell lines and again EE had the highest effect. The percentages of cell growth inhibition in HeLa, SiHa, and CaSki cells treated with EE at 24 h at 50 µg/mL were 45.79±4.11, 41.66±4.03, and 36.72±2.67, while they were 74.23±7.45, 62.31±5.97, and 54.23±5.04 at 150 µg/mL concentration. At 250 µg/mL concentration, the percentages of cell growth inhibition were 94.25 ±8.11, 90.02 ±8.67, and 85.43±6.21, respectively. The expression of apoptotic gene (Caspase 3, 9) and tumor guard gene (p53), as their proteins in Western blotting increased . However, anti-apoptotic BcL2 gene of all cell lines was decreased following treatment with extracts. In addition, the cell cycle analysis (CaSki cell) showed that treatment (EE) arrested at G2/M check point cell cycle. CONCLUSION: All extracts of this mushroom were active in arresting growth of three cell lines and EE had the highest effect, indicating that this mushroom can be a valuable source of anticancer agents.
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spelling pubmed-75734052020-10-30 Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines Ghosh, Swapan Kumar Sanyal, Tapojyoti Bera, Tanmay Asian Pac J Cancer Prev Research Article OBJECTIVE: In this study, we aimed to harness some solvent extracts of one wild mushroom Hexagonia glabra and test their anti-cancer activity against cervical human cell lines, namelyHeLa, SiHa, and CaSki. METHODS: It includes cell morphological study by microscope, nuclear morphology by DAPI staining under fluorescence microscopy, apoptosis assay by fluorescence technique, anti-proliferation by MTT assay and expression of apoptotic and anti-apoptotic genes by Western blotting and cell cycle analysis was done. RESULTS: The selected cervical cancer cells were treated separately with 150 µg/mL of three extracts, namely of ethanolic (EE), ethyl acetate (EAE), and water extract (WE) and exhibited features like round, shrink and dead. All extracts caused apoptosis in cell lines and EE had the highest effect in this regard. The percentages of apoptotic cells in HeLa, SiHa and CaSki, at the same concentration of EE were 79.23, 75.42, and 76.36% respectively. Cytotoxicity assay showed that all three extracts (50 – 250 μg/mL) were potent for inhibition of cell growth of three cell lines and again EE had the highest effect. The percentages of cell growth inhibition in HeLa, SiHa, and CaSki cells treated with EE at 24 h at 50 µg/mL were 45.79±4.11, 41.66±4.03, and 36.72±2.67, while they were 74.23±7.45, 62.31±5.97, and 54.23±5.04 at 150 µg/mL concentration. At 250 µg/mL concentration, the percentages of cell growth inhibition were 94.25 ±8.11, 90.02 ±8.67, and 85.43±6.21, respectively. The expression of apoptotic gene (Caspase 3, 9) and tumor guard gene (p53), as their proteins in Western blotting increased . However, anti-apoptotic BcL2 gene of all cell lines was decreased following treatment with extracts. In addition, the cell cycle analysis (CaSki cell) showed that treatment (EE) arrested at G2/M check point cell cycle. CONCLUSION: All extracts of this mushroom were active in arresting growth of three cell lines and EE had the highest effect, indicating that this mushroom can be a valuable source of anticancer agents. West Asia Organization for Cancer Prevention 2020-07 /pmc/articles/PMC7573405/ /pubmed/32711423 http://dx.doi.org/10.31557/APJCP.2020.21.7.1977 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ghosh, Swapan Kumar
Sanyal, Tapojyoti
Bera, Tanmay
Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title_full Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title_fullStr Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title_full_unstemmed Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title_short Anticancer Activity of Solvent Extracts of Hexogonia glabra against Cervical Cancer Cell Lines
title_sort anticancer activity of solvent extracts of hexogonia glabra against cervical cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573405/
https://www.ncbi.nlm.nih.gov/pubmed/32711423
http://dx.doi.org/10.31557/APJCP.2020.21.7.1977
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