Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions

[Image: see text] The formation of cytomimetic protocells that capture the physicochemical aspects of living cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded cytoplasm in liposome-based protocells and studied the kinetics of cell-free gene expression in these...

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Detalles Bibliográficos
Autores principales: Vibhute, Mahesh A., Schaap, Mark H., Maas, Roel J. M., Nelissen, Frank H. T., Spruijt, Evan, Heus, Hans A., Hansen, Maike M. K., Huck, Wilhelm T. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7573978/
https://www.ncbi.nlm.nih.gov/pubmed/32976714
http://dx.doi.org/10.1021/acssynbio.0c00330

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