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High Smad7 in the early post-operative recurrence of Crohn’s disease
BACKGROUND: In Crohn’s disease (CD), one of the major inflammatory bowel disease (IBD) in human beings, there is over-expression of Smad7, an intracellular inhibitor of the suppressive cytokine TGF-β1. The aim of this study was to assess whether Smad7 over-expression occurs in the early and/or late...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574182/ https://www.ncbi.nlm.nih.gov/pubmed/33076905 http://dx.doi.org/10.1186/s12967-020-02558-8 |
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author | Zorzi, Francesca Calabrese, Emma Di Fusco, Davide De Cristofaro, Elena Biancone, Livia Casella, Sara Palmieri, Giampiero Monteleone, Giovanni |
author_facet | Zorzi, Francesca Calabrese, Emma Di Fusco, Davide De Cristofaro, Elena Biancone, Livia Casella, Sara Palmieri, Giampiero Monteleone, Giovanni |
author_sort | Zorzi, Francesca |
collection | PubMed |
description | BACKGROUND: In Crohn’s disease (CD), one of the major inflammatory bowel disease (IBD) in human beings, there is over-expression of Smad7, an intracellular inhibitor of the suppressive cytokine TGF-β1. The aim of this study was to assess whether Smad7 over-expression occurs in the early and/or late phases of CD. METHODS: Mucosal samples were taken from the neo-terminal ileum of CD patients undergoing ileocolonic resection, with or without (early CD) post-operative endoscopic recurrence, and terminal ileum of CD patients with long-standing disease undergoing intestinal resection (late CD). Smad7 was examined by immunohistochemistry and cytokine expression was analysed by flow-cytometry. RESULTS: Before the appearance of endoscopic lesions, the mucosa of the neo-terminal ileum contained high number of Smad7-expressing cells in both the epithelial and lamina propria compartments. Transition from this stage to endoscopic recurrence was marked by persistence of high number of Smad7-positive cells, which reduced significantly in the late stages of the disease, where Smad7 expression remained, however, greater than that seen in normal controls. In samples with early lesions, Smad7 expression positively correlated with the number of interferon-γ-secreting cells. CONCLUSIONS: Smad7 induction is an early event in the inflammatory sequence occurring in CD, thus suggesting that knockdown of Smad7 can help prevent post-operative recurrence. |
format | Online Article Text |
id | pubmed-7574182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75741822020-10-20 High Smad7 in the early post-operative recurrence of Crohn’s disease Zorzi, Francesca Calabrese, Emma Di Fusco, Davide De Cristofaro, Elena Biancone, Livia Casella, Sara Palmieri, Giampiero Monteleone, Giovanni J Transl Med Research BACKGROUND: In Crohn’s disease (CD), one of the major inflammatory bowel disease (IBD) in human beings, there is over-expression of Smad7, an intracellular inhibitor of the suppressive cytokine TGF-β1. The aim of this study was to assess whether Smad7 over-expression occurs in the early and/or late phases of CD. METHODS: Mucosal samples were taken from the neo-terminal ileum of CD patients undergoing ileocolonic resection, with or without (early CD) post-operative endoscopic recurrence, and terminal ileum of CD patients with long-standing disease undergoing intestinal resection (late CD). Smad7 was examined by immunohistochemistry and cytokine expression was analysed by flow-cytometry. RESULTS: Before the appearance of endoscopic lesions, the mucosa of the neo-terminal ileum contained high number of Smad7-expressing cells in both the epithelial and lamina propria compartments. Transition from this stage to endoscopic recurrence was marked by persistence of high number of Smad7-positive cells, which reduced significantly in the late stages of the disease, where Smad7 expression remained, however, greater than that seen in normal controls. In samples with early lesions, Smad7 expression positively correlated with the number of interferon-γ-secreting cells. CONCLUSIONS: Smad7 induction is an early event in the inflammatory sequence occurring in CD, thus suggesting that knockdown of Smad7 can help prevent post-operative recurrence. BioMed Central 2020-10-19 /pmc/articles/PMC7574182/ /pubmed/33076905 http://dx.doi.org/10.1186/s12967-020-02558-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zorzi, Francesca Calabrese, Emma Di Fusco, Davide De Cristofaro, Elena Biancone, Livia Casella, Sara Palmieri, Giampiero Monteleone, Giovanni High Smad7 in the early post-operative recurrence of Crohn’s disease |
title | High Smad7 in the early post-operative recurrence of Crohn’s disease |
title_full | High Smad7 in the early post-operative recurrence of Crohn’s disease |
title_fullStr | High Smad7 in the early post-operative recurrence of Crohn’s disease |
title_full_unstemmed | High Smad7 in the early post-operative recurrence of Crohn’s disease |
title_short | High Smad7 in the early post-operative recurrence of Crohn’s disease |
title_sort | high smad7 in the early post-operative recurrence of crohn’s disease |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574182/ https://www.ncbi.nlm.nih.gov/pubmed/33076905 http://dx.doi.org/10.1186/s12967-020-02558-8 |
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