High throughput single-cell detection of multiplex CRISPR-edited gene modifications

CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered...

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Autores principales: ten Hacken, Elisa, Clement, Kendell, Li, Shuqiang, Hernández-Sánchez, María, Redd, Robert, Wang, Shu, Ruff, David, Gruber, Michaela, Baranowski, Kaitlyn, Jacob, Jose, Flynn, James, Jones, Keith W., Neuberg, Donna, Livak, Kenneth J., Pinello, Luca, Wu, Catherine J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574538/
https://www.ncbi.nlm.nih.gov/pubmed/33081820
http://dx.doi.org/10.1186/s13059-020-02174-1
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author ten Hacken, Elisa
Clement, Kendell
Li, Shuqiang
Hernández-Sánchez, María
Redd, Robert
Wang, Shu
Ruff, David
Gruber, Michaela
Baranowski, Kaitlyn
Jacob, Jose
Flynn, James
Jones, Keith W.
Neuberg, Donna
Livak, Kenneth J.
Pinello, Luca
Wu, Catherine J.
author_facet ten Hacken, Elisa
Clement, Kendell
Li, Shuqiang
Hernández-Sánchez, María
Redd, Robert
Wang, Shu
Ruff, David
Gruber, Michaela
Baranowski, Kaitlyn
Jacob, Jose
Flynn, James
Jones, Keith W.
Neuberg, Donna
Livak, Kenneth J.
Pinello, Luca
Wu, Catherine J.
author_sort ten Hacken, Elisa
collection PubMed
description CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution.
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spelling pubmed-75745382020-10-21 High throughput single-cell detection of multiplex CRISPR-edited gene modifications ten Hacken, Elisa Clement, Kendell Li, Shuqiang Hernández-Sánchez, María Redd, Robert Wang, Shu Ruff, David Gruber, Michaela Baranowski, Kaitlyn Jacob, Jose Flynn, James Jones, Keith W. Neuberg, Donna Livak, Kenneth J. Pinello, Luca Wu, Catherine J. Genome Biol Short Report CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach reliably quantifies mutational co-occurrences, zygosity status, and the occurrence of Cas9 edits at single-cell resolution. BioMed Central 2020-10-20 /pmc/articles/PMC7574538/ /pubmed/33081820 http://dx.doi.org/10.1186/s13059-020-02174-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
ten Hacken, Elisa
Clement, Kendell
Li, Shuqiang
Hernández-Sánchez, María
Redd, Robert
Wang, Shu
Ruff, David
Gruber, Michaela
Baranowski, Kaitlyn
Jacob, Jose
Flynn, James
Jones, Keith W.
Neuberg, Donna
Livak, Kenneth J.
Pinello, Luca
Wu, Catherine J.
High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title_full High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title_fullStr High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title_full_unstemmed High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title_short High throughput single-cell detection of multiplex CRISPR-edited gene modifications
title_sort high throughput single-cell detection of multiplex crispr-edited gene modifications
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7574538/
https://www.ncbi.nlm.nih.gov/pubmed/33081820
http://dx.doi.org/10.1186/s13059-020-02174-1
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