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Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research

The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has...

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Autores principales: Tuck, C. J., De Palma, G., Takami, K., Brant, B., Caminero, A., Reed, D. E., Muir, J. G., Gibson, P. R., Winterborn, A., Verdu, E. F., Bercik, P., Vanner, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575541/
https://www.ncbi.nlm.nih.gov/pubmed/33082369
http://dx.doi.org/10.1038/s41598-020-74460-8
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author Tuck, C. J.
De Palma, G.
Takami, K.
Brant, B.
Caminero, A.
Reed, D. E.
Muir, J. G.
Gibson, P. R.
Winterborn, A.
Verdu, E. F.
Bercik, P.
Vanner, S.
author_facet Tuck, C. J.
De Palma, G.
Takami, K.
Brant, B.
Caminero, A.
Reed, D. E.
Muir, J. G.
Gibson, P. R.
Winterborn, A.
Verdu, E. F.
Bercik, P.
Vanner, S.
author_sort Tuck, C. J.
collection PubMed
description The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dietary impact on cecal microbiota, short- and branched-chain fatty acid profiles was evaluated. The gut microbiota composition differed significantly between diets and sexes, with significantly different clusters in β-diversity. Total BCFA were highest (p = 0.01) and SCFA were lowest (p = 0.03) in mice fed a diet lower in FODMAPs and gluten. These results suggest that nutritional composition of commercially available rodent diets impact gut microbiota profiles and fermentation patterns, with major implications for the reproducibility of results across laboratories. However, further studies are required to elucidate the specific dietary factors driving these changes.
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spelling pubmed-75755412020-10-21 Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research Tuck, C. J. De Palma, G. Takami, K. Brant, B. Caminero, A. Reed, D. E. Muir, J. G. Gibson, P. R. Winterborn, A. Verdu, E. F. Bercik, P. Vanner, S. Sci Rep Article The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dietary impact on cecal microbiota, short- and branched-chain fatty acid profiles was evaluated. The gut microbiota composition differed significantly between diets and sexes, with significantly different clusters in β-diversity. Total BCFA were highest (p = 0.01) and SCFA were lowest (p = 0.03) in mice fed a diet lower in FODMAPs and gluten. These results suggest that nutritional composition of commercially available rodent diets impact gut microbiota profiles and fermentation patterns, with major implications for the reproducibility of results across laboratories. However, further studies are required to elucidate the specific dietary factors driving these changes. Nature Publishing Group UK 2020-10-20 /pmc/articles/PMC7575541/ /pubmed/33082369 http://dx.doi.org/10.1038/s41598-020-74460-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tuck, C. J.
De Palma, G.
Takami, K.
Brant, B.
Caminero, A.
Reed, D. E.
Muir, J. G.
Gibson, P. R.
Winterborn, A.
Verdu, E. F.
Bercik, P.
Vanner, S.
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title_full Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title_fullStr Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title_full_unstemmed Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title_short Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
title_sort nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575541/
https://www.ncbi.nlm.nih.gov/pubmed/33082369
http://dx.doi.org/10.1038/s41598-020-74460-8
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