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A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl

3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bi...

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Autores principales: Zhou, Miaomiao, Lee, Bae Hoon, Tan, Lay Poh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Whioce Publishing Pte. Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575630/
https://www.ncbi.nlm.nih.gov/pubmed/33094187
http://dx.doi.org/10.18063/IJB.2017.02.003
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author Zhou, Miaomiao
Lee, Bae Hoon
Tan, Lay Poh
author_facet Zhou, Miaomiao
Lee, Bae Hoon
Tan, Lay Poh
author_sort Zhou, Miaomiao
collection PubMed
description 3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca(2+)-independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy.
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spelling pubmed-75756302020-10-21 A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl Zhou, Miaomiao Lee, Bae Hoon Tan, Lay Poh Int J Bioprint Research Article 3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca(2+)-independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy. Whioce Publishing Pte. Ltd. 2017-07-11 /pmc/articles/PMC7575630/ /pubmed/33094187 http://dx.doi.org/10.18063/IJB.2017.02.003 Text en Copyright: © 2017 Zhou, et al. http://creativecommons.org/licenses/cc-by-nc/4.0/ This is an open-access article distributed under the terms of the Attribution-NonCommercial 4.0 International 4.0 (CC BY-NC 4.0), which permits all non-commercial use, distribution, and reproduction in any medium provided the original work is properly cited.
spellingShingle Research Article
Zhou, Miaomiao
Lee, Bae Hoon
Tan, Lay Poh
A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title_full A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title_fullStr A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title_full_unstemmed A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title_short A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
title_sort dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575630/
https://www.ncbi.nlm.nih.gov/pubmed/33094187
http://dx.doi.org/10.18063/IJB.2017.02.003
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