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Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria

Previous reports demonstrated the utility of systemic application of growth hormone (GH) in the treatment of bone defects. Very few studies correlated bone repair efficacy with hepatic and renal side effects promoted by locally-delivered GH. The objectives of this study were to assess the bone repai...

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Autores principales: Chaves, Luis Henrique, Giovanini, Allan Fernando, Zielak, Joao Cesar, Scariot, Rafaela, Gonzaga, Carla Castiglia, Storrer, Carmen Lucia Mueller, Khajotia, Sharukh Soli, Esteban Florez, Fernando Luis, Deliberador, Tatiana Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575886/
https://www.ncbi.nlm.nih.gov/pubmed/33102851
http://dx.doi.org/10.1016/j.heliyon.2020.e05226
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author Chaves, Luis Henrique
Giovanini, Allan Fernando
Zielak, Joao Cesar
Scariot, Rafaela
Gonzaga, Carla Castiglia
Storrer, Carmen Lucia Mueller
Khajotia, Sharukh Soli
Esteban Florez, Fernando Luis
Deliberador, Tatiana Miranda
author_facet Chaves, Luis Henrique
Giovanini, Allan Fernando
Zielak, Joao Cesar
Scariot, Rafaela
Gonzaga, Carla Castiglia
Storrer, Carmen Lucia Mueller
Khajotia, Sharukh Soli
Esteban Florez, Fernando Luis
Deliberador, Tatiana Miranda
author_sort Chaves, Luis Henrique
collection PubMed
description Previous reports demonstrated the utility of systemic application of growth hormone (GH) in the treatment of bone defects. Very few studies correlated bone repair efficacy with hepatic and renal side effects promoted by locally-delivered GH. The objectives of this study were to assess the bone repair properties along with hepatic and renal adverse effects promoted by local application of GH in a rat model. Thirty-two rats were randomly divided (4 groups; n = 8/group), as follows: (i) AB (autogenous bone + local application of saline solution [SS]), (ii) AB+ (autogenous bone + SS local application + SS irrigation), (iii) AB/GH+ (autogenous bone + SS local application + GH irrigation) and (iv) AB/GHL+ (autogenous bone + GH local application + GH irrigation). Critical-sized defects (diameter = 5.0 mm) were surgically created by a single operator in the calvaria of rats. Defects were filled with ground autogenous bone. Defects pertaining to AB+ and AB/GH+ received a mixture of autogenous bone and a SS-saturated (0.02 mL) collagen sponge covered with bovine cortical membrane. Defects in group AB/GHL+, were filled with the same biomaterials saturated with GH (0.02 mL). SS (0.1 mL) or GH (0.1 mL, equivalent to 0.4 IU) were applied locally on alternate days (8 weeks) in animals in groups AB, AB+ and AB/GH+ or AB/GHL+, respectively. Bone repair properties was determined in hematoxylin/eosin-stained slices using traditional histologic and histomorphometric techniques along with optical microscopy and digital image analysis. Statistical differences among groups was determined using Kruskal-Wallis and Tukey post hoc tests (α = 0.05). Histology results indicated that AB and AB+ displayed greater presence of autogenous bone as compared to AB/GH+ and AB/GHL+. Histomorphometric results indicated significantly higher osteoid matrix formation in AB and AB+ when compared to AB/GHL+ (p = 0.009). Kidneys and livers were found to have their glomeruli preserved in AB and AB+. Strong glomeruli necrosis and large areas of protein deposition were found in AB/GH+. Abnormal small-sized glomeruli were found in AB/GHL+. The utilization of autogenous bone graft associated with local application and irrigation with GH was shown to not improve the bone repair in calvarial critical-sized defects in a rat model.
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spelling pubmed-75758862020-10-23 Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria Chaves, Luis Henrique Giovanini, Allan Fernando Zielak, Joao Cesar Scariot, Rafaela Gonzaga, Carla Castiglia Storrer, Carmen Lucia Mueller Khajotia, Sharukh Soli Esteban Florez, Fernando Luis Deliberador, Tatiana Miranda Heliyon Research Article Previous reports demonstrated the utility of systemic application of growth hormone (GH) in the treatment of bone defects. Very few studies correlated bone repair efficacy with hepatic and renal side effects promoted by locally-delivered GH. The objectives of this study were to assess the bone repair properties along with hepatic and renal adverse effects promoted by local application of GH in a rat model. Thirty-two rats were randomly divided (4 groups; n = 8/group), as follows: (i) AB (autogenous bone + local application of saline solution [SS]), (ii) AB+ (autogenous bone + SS local application + SS irrigation), (iii) AB/GH+ (autogenous bone + SS local application + GH irrigation) and (iv) AB/GHL+ (autogenous bone + GH local application + GH irrigation). Critical-sized defects (diameter = 5.0 mm) were surgically created by a single operator in the calvaria of rats. Defects were filled with ground autogenous bone. Defects pertaining to AB+ and AB/GH+ received a mixture of autogenous bone and a SS-saturated (0.02 mL) collagen sponge covered with bovine cortical membrane. Defects in group AB/GHL+, were filled with the same biomaterials saturated with GH (0.02 mL). SS (0.1 mL) or GH (0.1 mL, equivalent to 0.4 IU) were applied locally on alternate days (8 weeks) in animals in groups AB, AB+ and AB/GH+ or AB/GHL+, respectively. Bone repair properties was determined in hematoxylin/eosin-stained slices using traditional histologic and histomorphometric techniques along with optical microscopy and digital image analysis. Statistical differences among groups was determined using Kruskal-Wallis and Tukey post hoc tests (α = 0.05). Histology results indicated that AB and AB+ displayed greater presence of autogenous bone as compared to AB/GH+ and AB/GHL+. Histomorphometric results indicated significantly higher osteoid matrix formation in AB and AB+ when compared to AB/GHL+ (p = 0.009). Kidneys and livers were found to have their glomeruli preserved in AB and AB+. Strong glomeruli necrosis and large areas of protein deposition were found in AB/GH+. Abnormal small-sized glomeruli were found in AB/GHL+. The utilization of autogenous bone graft associated with local application and irrigation with GH was shown to not improve the bone repair in calvarial critical-sized defects in a rat model. Elsevier 2020-10-16 /pmc/articles/PMC7575886/ /pubmed/33102851 http://dx.doi.org/10.1016/j.heliyon.2020.e05226 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Chaves, Luis Henrique
Giovanini, Allan Fernando
Zielak, Joao Cesar
Scariot, Rafaela
Gonzaga, Carla Castiglia
Storrer, Carmen Lucia Mueller
Khajotia, Sharukh Soli
Esteban Florez, Fernando Luis
Deliberador, Tatiana Miranda
Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title_full Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title_fullStr Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title_full_unstemmed Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title_short Growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
title_sort growth hormone effects on healing efficacy, bone resorption and renal morphology of rats: histological and histometric study in rat calvaria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575886/
https://www.ncbi.nlm.nih.gov/pubmed/33102851
http://dx.doi.org/10.1016/j.heliyon.2020.e05226
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