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The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity

BACKGROUND: The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an importan...

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Autores principales: Li, Min-Jie, Wang, Xi, Chen, Ying, Li, Guang-Jian, Zhao, Guang-Qiang, Xiang, Bing-Quan, Wei, Xue-Qiang, Lei, Yu-Jie, Huang, Yun-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575940/
https://www.ncbi.nlm.nih.gov/pubmed/33145295
http://dx.doi.org/10.21037/atm-20-4437
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author Li, Min-Jie
Wang, Xi
Chen, Ying
Li, Guang-Jian
Zhao, Guang-Qiang
Xiang, Bing-Quan
Wei, Xue-Qiang
Lei, Yu-Jie
Huang, Yun-Chao
author_facet Li, Min-Jie
Wang, Xi
Chen, Ying
Li, Guang-Jian
Zhao, Guang-Qiang
Xiang, Bing-Quan
Wei, Xue-Qiang
Lei, Yu-Jie
Huang, Yun-Chao
author_sort Li, Min-Jie
collection PubMed
description BACKGROUND: The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an important group of immune down-regulation factors. Our study aims to investigate the effect of TGF-β1 on the morphology and cellular immune function of A549 and peripheral blood mononuclear cells (PBMCs). METHODS: A549 cell line was cultured, PBMCs were cultured with different concentrations of TGF-β1, and the morphology of A549 cells and PBMCs were seen. The levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IFN-γ, and TNF and the numbers of CD3, CD4, CD8, CD4/CD8, and CD3 CD25 and CD4 CD25 in PBMCs were detected. RESULTS: During co-culture of A549 with PBMCs, TGF-β1 can induced A549 showing epithelial-to-mesenchymal transition, enhanced its ability of migration and infiltration. Simultaneously, TGF-β1 can depressing the growth and proliferation of PBMCs, inhibiting T-cell activation, and accelerating the PBMCs apoptosis. TGF-β1 can inhibits A549 Th1 related-cytokines, enhance Th2 related-cytokines, cause the disorder of Th1/Th2, resulting in the Th1 cellular dominate immunity decline. CONCLUSIONS: TGF-β1 may affect the secretion of related cytokines, hinder the activation of T lymphocytes, destroy the immune surveillance and killing effect of the body, and thus inhibit the cellular immunity.
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spelling pubmed-75759402020-11-02 The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity Li, Min-Jie Wang, Xi Chen, Ying Li, Guang-Jian Zhao, Guang-Qiang Xiang, Bing-Quan Wei, Xue-Qiang Lei, Yu-Jie Huang, Yun-Chao Ann Transl Med Original Article BACKGROUND: The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an important group of immune down-regulation factors. Our study aims to investigate the effect of TGF-β1 on the morphology and cellular immune function of A549 and peripheral blood mononuclear cells (PBMCs). METHODS: A549 cell line was cultured, PBMCs were cultured with different concentrations of TGF-β1, and the morphology of A549 cells and PBMCs were seen. The levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IFN-γ, and TNF and the numbers of CD3, CD4, CD8, CD4/CD8, and CD3 CD25 and CD4 CD25 in PBMCs were detected. RESULTS: During co-culture of A549 with PBMCs, TGF-β1 can induced A549 showing epithelial-to-mesenchymal transition, enhanced its ability of migration and infiltration. Simultaneously, TGF-β1 can depressing the growth and proliferation of PBMCs, inhibiting T-cell activation, and accelerating the PBMCs apoptosis. TGF-β1 can inhibits A549 Th1 related-cytokines, enhance Th2 related-cytokines, cause the disorder of Th1/Th2, resulting in the Th1 cellular dominate immunity decline. CONCLUSIONS: TGF-β1 may affect the secretion of related cytokines, hinder the activation of T lymphocytes, destroy the immune surveillance and killing effect of the body, and thus inhibit the cellular immunity. AME Publishing Company 2020-09 /pmc/articles/PMC7575940/ /pubmed/33145295 http://dx.doi.org/10.21037/atm-20-4437 Text en 2020 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Li, Min-Jie
Wang, Xi
Chen, Ying
Li, Guang-Jian
Zhao, Guang-Qiang
Xiang, Bing-Quan
Wei, Xue-Qiang
Lei, Yu-Jie
Huang, Yun-Chao
The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title_full The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title_fullStr The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title_full_unstemmed The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title_short The influences of TGF-β1 upon the human adenocarcinoma cell of lung A549 and cellular immunity
title_sort influences of tgf-β1 upon the human adenocarcinoma cell of lung a549 and cellular immunity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7575940/
https://www.ncbi.nlm.nih.gov/pubmed/33145295
http://dx.doi.org/10.21037/atm-20-4437
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