Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria

Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have unique and versatile metabolisms, offering unique opportunities for Synthetic Biology (SynBio). However, key genome editing and recombineering tools require optimization and large-scale multiplexing to unlock the f...

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Autores principales: Lammens, Eveline-Marie, Nikel, Pablo Ivan, Lavigne, Rob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576135/
https://www.ncbi.nlm.nih.gov/pubmed/33082347
http://dx.doi.org/10.1038/s41467-020-19124-x
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author Lammens, Eveline-Marie
Nikel, Pablo Ivan
Lavigne, Rob
author_facet Lammens, Eveline-Marie
Nikel, Pablo Ivan
Lavigne, Rob
author_sort Lammens, Eveline-Marie
collection PubMed
description Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have unique and versatile metabolisms, offering unique opportunities for Synthetic Biology (SynBio). However, key genome editing and recombineering tools require optimization and large-scale multiplexing to unlock the full SynBio potential of these bacteria. In addition, the limited availability of a set of characterized, species-specific biological parts hampers the construction of reliable genetic circuitry. Mining of currently available, diverse bacteriophages could complete the SynBio toolbox, as they constitute an unexplored treasure trove for fully adapted metabolic modulators and orthogonally-functioning parts, driven by the longstanding co-evolution between phage and host.
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spelling pubmed-75761352020-10-29 Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria Lammens, Eveline-Marie Nikel, Pablo Ivan Lavigne, Rob Nat Commun Perspective Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have unique and versatile metabolisms, offering unique opportunities for Synthetic Biology (SynBio). However, key genome editing and recombineering tools require optimization and large-scale multiplexing to unlock the full SynBio potential of these bacteria. In addition, the limited availability of a set of characterized, species-specific biological parts hampers the construction of reliable genetic circuitry. Mining of currently available, diverse bacteriophages could complete the SynBio toolbox, as they constitute an unexplored treasure trove for fully adapted metabolic modulators and orthogonally-functioning parts, driven by the longstanding co-evolution between phage and host. Nature Publishing Group UK 2020-10-20 /pmc/articles/PMC7576135/ /pubmed/33082347 http://dx.doi.org/10.1038/s41467-020-19124-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Perspective
Lammens, Eveline-Marie
Nikel, Pablo Ivan
Lavigne, Rob
Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title_full Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title_fullStr Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title_full_unstemmed Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title_short Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
title_sort exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576135/
https://www.ncbi.nlm.nih.gov/pubmed/33082347
http://dx.doi.org/10.1038/s41467-020-19124-x
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