Construction and identification of influenza plasmid pool imparting high yields to candidate vaccine viruses in Vero cell at low temperature

We generated plasmid pools for the rapid preparation of candidate vaccine strains, which could grow in the Vero cells at low temperature. Firstly, we cloned in the pHW2000 plasmid each of the eight gene segments (PB2, PB1, PA, hemagglutinin [HA], neuraminidase [NA], NS, NP, M) of two master donor st...

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Detalles Bibliográficos
Autores principales: Liu, Ze, Geng, Xingliang, Cui, Zhaohai, Li, Weidong, Ou, Xia, Liao, Guoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576294/
https://www.ncbi.nlm.nih.gov/pubmed/32902192
http://dx.doi.org/10.1111/jcmm.15672

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576294/
https://www.ncbi.nlm.nih.gov/pubmed/32902192
http://dx.doi.org/10.1111/jcmm.15672

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