Cargando…
Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis
Reduced activation of energy metabolism increases adiposity in humans and other mammals. Thus, exploring dietary and molecular mechanisms able to improve energy metabolism is of paramount medical importance because such mechanisms can be leveraged as a therapy for obesity and related disorders. Here...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Diabetes Association
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576563/ https://www.ncbi.nlm.nih.gov/pubmed/32778569 http://dx.doi.org/10.2337/db20-0489 |
_version_ | 1783598041073188864 |
---|---|
author | Ruocco, Chiara Ragni, Maurizio Rossi, Fabio Carullo, Pierluigi Ghini, Veronica Piscitelli, Fabiana Cutignano, Adele Manzo, Emiliano Ioris, Rafael Maciel Bontems, Franck Tedesco, Laura Greco, Carolina M. Pino, Annachiara Severi, Ilenia Liu, Dianxin Ceddia, Ryan P. Ponzoni, Luisa Tenori, Leonardo Rizzetto, Lisa Scholz, Matthias Tuohy, Kieran Bifari, Francesco Di Marzo, Vincenzo Luchinat, Claudio Carruba, Michele O. Cinti, Saverio Decimo, Ilaria Condorelli, Gianluigi Coppari, Roberto Collins, Sheila Valerio, Alessandra Nisoli, Enzo |
author_facet | Ruocco, Chiara Ragni, Maurizio Rossi, Fabio Carullo, Pierluigi Ghini, Veronica Piscitelli, Fabiana Cutignano, Adele Manzo, Emiliano Ioris, Rafael Maciel Bontems, Franck Tedesco, Laura Greco, Carolina M. Pino, Annachiara Severi, Ilenia Liu, Dianxin Ceddia, Ryan P. Ponzoni, Luisa Tenori, Leonardo Rizzetto, Lisa Scholz, Matthias Tuohy, Kieran Bifari, Francesco Di Marzo, Vincenzo Luchinat, Claudio Carruba, Michele O. Cinti, Saverio Decimo, Ilaria Condorelli, Gianluigi Coppari, Roberto Collins, Sheila Valerio, Alessandra Nisoli, Enzo |
author_sort | Ruocco, Chiara |
collection | PubMed |
description | Reduced activation of energy metabolism increases adiposity in humans and other mammals. Thus, exploring dietary and molecular mechanisms able to improve energy metabolism is of paramount medical importance because such mechanisms can be leveraged as a therapy for obesity and related disorders. Here, we show that a designer protein-deprived diet enriched in free essential amino acids can 1) promote the brown fat thermogenic program and fatty acid oxidation, 2) stimulate uncoupling protein 1 (UCP1)-independent respiration in subcutaneous white fat, 3) change the gut microbiota composition, and 4) prevent and reverse obesity and dysregulated glucose homeostasis in multiple mouse models, prolonging the healthy life span. These effects are independent of unbalanced amino acid ratio, energy consumption, and intestinal calorie absorption. A brown fat-specific activation of the mechanistic target of rapamycin complex 1 seems involved in the diet-induced beneficial effects, as also strengthened by in vitro experiments. Hence, our results suggest that brown and white fat may be targets of specific amino acids to control UCP1-dependent and -independent thermogenesis, thereby contributing to the improvement of metabolic health. |
format | Online Article Text |
id | pubmed-7576563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-75765632020-11-02 Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis Ruocco, Chiara Ragni, Maurizio Rossi, Fabio Carullo, Pierluigi Ghini, Veronica Piscitelli, Fabiana Cutignano, Adele Manzo, Emiliano Ioris, Rafael Maciel Bontems, Franck Tedesco, Laura Greco, Carolina M. Pino, Annachiara Severi, Ilenia Liu, Dianxin Ceddia, Ryan P. Ponzoni, Luisa Tenori, Leonardo Rizzetto, Lisa Scholz, Matthias Tuohy, Kieran Bifari, Francesco Di Marzo, Vincenzo Luchinat, Claudio Carruba, Michele O. Cinti, Saverio Decimo, Ilaria Condorelli, Gianluigi Coppari, Roberto Collins, Sheila Valerio, Alessandra Nisoli, Enzo Diabetes Obesity Studies Reduced activation of energy metabolism increases adiposity in humans and other mammals. Thus, exploring dietary and molecular mechanisms able to improve energy metabolism is of paramount medical importance because such mechanisms can be leveraged as a therapy for obesity and related disorders. Here, we show that a designer protein-deprived diet enriched in free essential amino acids can 1) promote the brown fat thermogenic program and fatty acid oxidation, 2) stimulate uncoupling protein 1 (UCP1)-independent respiration in subcutaneous white fat, 3) change the gut microbiota composition, and 4) prevent and reverse obesity and dysregulated glucose homeostasis in multiple mouse models, prolonging the healthy life span. These effects are independent of unbalanced amino acid ratio, energy consumption, and intestinal calorie absorption. A brown fat-specific activation of the mechanistic target of rapamycin complex 1 seems involved in the diet-induced beneficial effects, as also strengthened by in vitro experiments. Hence, our results suggest that brown and white fat may be targets of specific amino acids to control UCP1-dependent and -independent thermogenesis, thereby contributing to the improvement of metabolic health. American Diabetes Association 2020-11 2020-08-10 /pmc/articles/PMC7576563/ /pubmed/32778569 http://dx.doi.org/10.2337/db20-0489 Text en © 2020 by the American Diabetes Association https://www.diabetesjournals.org/content/licenseReaders may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at https://www.diabetesjournals.org/content/license. |
spellingShingle | Obesity Studies Ruocco, Chiara Ragni, Maurizio Rossi, Fabio Carullo, Pierluigi Ghini, Veronica Piscitelli, Fabiana Cutignano, Adele Manzo, Emiliano Ioris, Rafael Maciel Bontems, Franck Tedesco, Laura Greco, Carolina M. Pino, Annachiara Severi, Ilenia Liu, Dianxin Ceddia, Ryan P. Ponzoni, Luisa Tenori, Leonardo Rizzetto, Lisa Scholz, Matthias Tuohy, Kieran Bifari, Francesco Di Marzo, Vincenzo Luchinat, Claudio Carruba, Michele O. Cinti, Saverio Decimo, Ilaria Condorelli, Gianluigi Coppari, Roberto Collins, Sheila Valerio, Alessandra Nisoli, Enzo Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title | Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title_full | Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title_fullStr | Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title_full_unstemmed | Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title_short | Manipulation of Dietary Amino Acids Prevents and Reverses Obesity in Mice Through Multiple Mechanisms That Modulate Energy Homeostasis |
title_sort | manipulation of dietary amino acids prevents and reverses obesity in mice through multiple mechanisms that modulate energy homeostasis |
topic | Obesity Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576563/ https://www.ncbi.nlm.nih.gov/pubmed/32778569 http://dx.doi.org/10.2337/db20-0489 |
work_keys_str_mv | AT ruoccochiara manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT ragnimaurizio manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT rossifabio manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT carullopierluigi manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT ghiniveronica manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT piscitellifabiana manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT cutignanoadele manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT manzoemiliano manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT iorisrafaelmaciel manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT bontemsfranck manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT tedescolaura manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT grecocarolinam manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT pinoannachiara manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT severiilenia manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT liudianxin manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT ceddiaryanp manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT ponzoniluisa manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT tenorileonardo manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT rizzettolisa manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT scholzmatthias manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT tuohykieran manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT bifarifrancesco manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT dimarzovincenzo manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT luchinatclaudio manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT carrubamicheleo manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT cintisaverio manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT decimoilaria manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT condorelligianluigi manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT coppariroberto manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT collinssheila manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT valerioalessandra manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis AT nisolienzo manipulationofdietaryaminoacidspreventsandreversesobesityinmicethroughmultiplemechanismsthatmodulateenergyhomeostasis |