Tools and Approaches for Studying Microglia In vivo

Microglia are specialized resident macrophages of the central nervous system (CNS) that have important functions during neurodevelopment, homeostasis and disease. This mini-review provides an overview of the current tools and approaches for studying microglia in vivo. We focus on tools for labeling...

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Detalles Bibliográficos
Autores principales: Eme-Scolan, Elisa, Dando, Samantha J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576994/
https://www.ncbi.nlm.nih.gov/pubmed/33117395
http://dx.doi.org/10.3389/fimmu.2020.583647
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author Eme-Scolan, Elisa
Dando, Samantha J.
author_facet Eme-Scolan, Elisa
Dando, Samantha J.
author_sort Eme-Scolan, Elisa
collection PubMed
description Microglia are specialized resident macrophages of the central nervous system (CNS) that have important functions during neurodevelopment, homeostasis and disease. This mini-review provides an overview of the current tools and approaches for studying microglia in vivo. We focus on tools for labeling microglia, highlighting the advantages and limitations of microglia markers/antibodies and reporter mice. We also discuss techniques for imaging microglia in situ, including in vivo live imaging of brain and retinal microglia. Finally, we review microglia depletion approaches and their use to investigate microglial function in CNS homeostasis and disease.
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spelling pubmed-75769942020-10-27 Tools and Approaches for Studying Microglia In vivo Eme-Scolan, Elisa Dando, Samantha J. Front Immunol Immunology Microglia are specialized resident macrophages of the central nervous system (CNS) that have important functions during neurodevelopment, homeostasis and disease. This mini-review provides an overview of the current tools and approaches for studying microglia in vivo. We focus on tools for labeling microglia, highlighting the advantages and limitations of microglia markers/antibodies and reporter mice. We also discuss techniques for imaging microglia in situ, including in vivo live imaging of brain and retinal microglia. Finally, we review microglia depletion approaches and their use to investigate microglial function in CNS homeostasis and disease. Frontiers Media S.A. 2020-10-07 /pmc/articles/PMC7576994/ /pubmed/33117395 http://dx.doi.org/10.3389/fimmu.2020.583647 Text en Copyright © 2020 Eme-Scolan and Dando. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Eme-Scolan, Elisa
Dando, Samantha J.
Tools and Approaches for Studying Microglia In vivo
title Tools and Approaches for Studying Microglia In vivo
title_full Tools and Approaches for Studying Microglia In vivo
title_fullStr Tools and Approaches for Studying Microglia In vivo
title_full_unstemmed Tools and Approaches for Studying Microglia In vivo
title_short Tools and Approaches for Studying Microglia In vivo
title_sort tools and approaches for studying microglia in vivo
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576994/
https://www.ncbi.nlm.nih.gov/pubmed/33117395
http://dx.doi.org/10.3389/fimmu.2020.583647
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