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In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2

SARS-CoV-2, which causes severe pneumonia epidemics, probably originated from Chinese horseshoe bats, but the intermediate and host range is still unknown. ACE2 is the entry receptor for SARS-CoV-2. The binding capacity of SARS-CoV-2 spike protein to ACE2 is the critical determinant of viral host ra...

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Autores principales: Bouricha, El Mehdi, Hakmi, Mohammed, Akachar, Jihane, Belyamani, Lahcen, Ibrahimi, Azeddine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577366/
https://www.ncbi.nlm.nih.gov/pubmed/33101829
http://dx.doi.org/10.1007/s13205-020-02471-3
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author Bouricha, El Mehdi
Hakmi, Mohammed
Akachar, Jihane
Belyamani, Lahcen
Ibrahimi, Azeddine
author_facet Bouricha, El Mehdi
Hakmi, Mohammed
Akachar, Jihane
Belyamani, Lahcen
Ibrahimi, Azeddine
author_sort Bouricha, El Mehdi
collection PubMed
description SARS-CoV-2, which causes severe pneumonia epidemics, probably originated from Chinese horseshoe bats, but the intermediate and host range is still unknown. ACE2 is the entry receptor for SARS-CoV-2. The binding capacity of SARS-CoV-2 spike protein to ACE2 is the critical determinant of viral host range and cross-species infection. Here, we used an in silico approach to predict the potential animals range with high susceptibility to SARS-CoV-2 by modelling and studying the Spike–ACE2 interaction of 22 domestic and wild animals. Our results showed that all studied animals are potentially susceptible to SARS-CoV-2 infection with a slight difference in the binding affinity and stability of their ACE2–RBD complexes. Furthermore, we identified a specific substitution of tyrosine to histidine at position 41 in ACE2 that likely reduces the affinity to SARS-CoV-2 in horses and greater horseshoe bats. These results may help to provide important insights into SARS-CoV-2 host range which will make it possible to control the spread of the virus and identify animal models that could be used for screening antiviral drugs or vaccine candidates against SARS-CoV-2.
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spelling pubmed-75773662020-10-22 In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2 Bouricha, El Mehdi Hakmi, Mohammed Akachar, Jihane Belyamani, Lahcen Ibrahimi, Azeddine 3 Biotech Original Article SARS-CoV-2, which causes severe pneumonia epidemics, probably originated from Chinese horseshoe bats, but the intermediate and host range is still unknown. ACE2 is the entry receptor for SARS-CoV-2. The binding capacity of SARS-CoV-2 spike protein to ACE2 is the critical determinant of viral host range and cross-species infection. Here, we used an in silico approach to predict the potential animals range with high susceptibility to SARS-CoV-2 by modelling and studying the Spike–ACE2 interaction of 22 domestic and wild animals. Our results showed that all studied animals are potentially susceptible to SARS-CoV-2 infection with a slight difference in the binding affinity and stability of their ACE2–RBD complexes. Furthermore, we identified a specific substitution of tyrosine to histidine at position 41 in ACE2 that likely reduces the affinity to SARS-CoV-2 in horses and greater horseshoe bats. These results may help to provide important insights into SARS-CoV-2 host range which will make it possible to control the spread of the virus and identify animal models that could be used for screening antiviral drugs or vaccine candidates against SARS-CoV-2. Springer International Publishing 2020-10-21 2020-11 /pmc/articles/PMC7577366/ /pubmed/33101829 http://dx.doi.org/10.1007/s13205-020-02471-3 Text en © King Abdulaziz City for Science and Technology 2020
spellingShingle Original Article
Bouricha, El Mehdi
Hakmi, Mohammed
Akachar, Jihane
Belyamani, Lahcen
Ibrahimi, Azeddine
In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title_full In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title_fullStr In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title_full_unstemmed In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title_short In silico analysis of ACE2 orthologues to predict animal host range with high susceptibility to SARS-CoV-2
title_sort in silico analysis of ace2 orthologues to predict animal host range with high susceptibility to sars-cov-2
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577366/
https://www.ncbi.nlm.nih.gov/pubmed/33101829
http://dx.doi.org/10.1007/s13205-020-02471-3
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