Cargando…

Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study

OBJECTIVES: This study aimed to investigate the immunological and bacterial profiles in pregnant women of Bafang-Cameroon. METHODS: Stool and midstream urine were cultured using specific culture media. The disk diffusion method was used for the antimicrobial susceptibility test. T-cell lymphocyte co...

Descripción completa

Detalles Bibliográficos
Autores principales: Ngalani, Ornella JT, Marbou, Wiliane JT, Mbaveng, Armelle Tsafack, Kuete, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Centers for Disease Control and Prevention 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577391/
https://www.ncbi.nlm.nih.gov/pubmed/33117637
http://dx.doi.org/10.24171/j.phrp.2020.11.5.08
_version_ 1783598185327886336
author Ngalani, Ornella JT
Marbou, Wiliane JT
Mbaveng, Armelle Tsafack
Kuete, Victor
author_facet Ngalani, Ornella JT
Marbou, Wiliane JT
Mbaveng, Armelle Tsafack
Kuete, Victor
author_sort Ngalani, Ornella JT
collection PubMed
description OBJECTIVES: This study aimed to investigate the immunological and bacterial profiles in pregnant women of Bafang-Cameroon. METHODS: Stool and midstream urine were cultured using specific culture media. The disk diffusion method was used for the antimicrobial susceptibility test. T-cell lymphocyte counts (CD3, CD4 and CD8), white blood cell counts, sensitive C-reactive protein, and interleukin-6, were measured by flow cytometry, optical detection, and the enzyme-linked immunosorbent assay solid phase direct sandwich method. RESULTS: Out of 700 participants, 71.43% were pregnant, and 28.57% were non-pregnant women. The mean age was 29.40 ± 8.27 and 27.41 ± 6.55 years in non-pregnant and pregnant women, respectively. CD4 T-cells were not significantly lower in pregnant women compared with non-pregnant women. There were 43.65% and 56.35% bacteria isolates obtained from urine and stool samples, respectively. Bacteria were mostly isolated in patients with a CD4 T-cell count between 461 and 806 cells/μL. Isolates of Klebsiella pneumoniae and Enterobacter aerogenes showed 100% resistance in non-pregnant women, however all isolated bacteria were shown to be multidrug resistant in pregnant women. Salmonella sp. (24.3%) and Escherichia coli (21.51%) showed an increase in multidrug resistant phenotypes in pregnant women. CONCLUSION: This study demonstrated that routine bacteriological analysis during pregnancy is necessary for their follow-up care.
format Online
Article
Text
id pubmed-7577391
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Korea Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-75773912020-10-27 Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study Ngalani, Ornella JT Marbou, Wiliane JT Mbaveng, Armelle Tsafack Kuete, Victor Osong Public Health Res Perspect Original Article OBJECTIVES: This study aimed to investigate the immunological and bacterial profiles in pregnant women of Bafang-Cameroon. METHODS: Stool and midstream urine were cultured using specific culture media. The disk diffusion method was used for the antimicrobial susceptibility test. T-cell lymphocyte counts (CD3, CD4 and CD8), white blood cell counts, sensitive C-reactive protein, and interleukin-6, were measured by flow cytometry, optical detection, and the enzyme-linked immunosorbent assay solid phase direct sandwich method. RESULTS: Out of 700 participants, 71.43% were pregnant, and 28.57% were non-pregnant women. The mean age was 29.40 ± 8.27 and 27.41 ± 6.55 years in non-pregnant and pregnant women, respectively. CD4 T-cells were not significantly lower in pregnant women compared with non-pregnant women. There were 43.65% and 56.35% bacteria isolates obtained from urine and stool samples, respectively. Bacteria were mostly isolated in patients with a CD4 T-cell count between 461 and 806 cells/μL. Isolates of Klebsiella pneumoniae and Enterobacter aerogenes showed 100% resistance in non-pregnant women, however all isolated bacteria were shown to be multidrug resistant in pregnant women. Salmonella sp. (24.3%) and Escherichia coli (21.51%) showed an increase in multidrug resistant phenotypes in pregnant women. CONCLUSION: This study demonstrated that routine bacteriological analysis during pregnancy is necessary for their follow-up care. Korea Centers for Disease Control and Prevention 2020-10 /pmc/articles/PMC7577391/ /pubmed/33117637 http://dx.doi.org/10.24171/j.phrp.2020.11.5.08 Text en Copyright ©2020, Korea Centers for Disease Control and Prevention http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Ngalani, Ornella JT
Marbou, Wiliane JT
Mbaveng, Armelle Tsafack
Kuete, Victor
Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title_full Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title_fullStr Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title_full_unstemmed Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title_short Immunological Profile and Bacterial Drug Resistance in Pregnant Women: A Cross Sectional Study
title_sort immunological profile and bacterial drug resistance in pregnant women: a cross sectional study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577391/
https://www.ncbi.nlm.nih.gov/pubmed/33117637
http://dx.doi.org/10.24171/j.phrp.2020.11.5.08
work_keys_str_mv AT ngalaniornellajt immunologicalprofileandbacterialdrugresistanceinpregnantwomenacrosssectionalstudy
AT marbouwilianejt immunologicalprofileandbacterialdrugresistanceinpregnantwomenacrosssectionalstudy
AT mbavengarmelletsafack immunologicalprofileandbacterialdrugresistanceinpregnantwomenacrosssectionalstudy
AT kuetevictor immunologicalprofileandbacterialdrugresistanceinpregnantwomenacrosssectionalstudy