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The relationship between the commensal microbiota levels and Crohn's disease activity
BACKGROUND AND AIM: Human gut microbiota play an important role in metabolism and host physiology. Perturbations of the gut microbial communities lead to the development of various diseases such as inflammatory bowel disease, celiac disease, allergic diseases, and metabolic diseases. Crohn's di...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Publishing Asia Pty Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578322/ https://www.ncbi.nlm.nih.gov/pubmed/33102745 http://dx.doi.org/10.1002/jgh3.12338 |
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author | de Alencar Junior, Hagamenon Paiotti, Ana Paula Ribeiro de Araújo Filho, Humberto Bezerra Oshima, Celina Tizuko Fujiyama Miszputen, Sender Jankiel Ambrogini‐Júnior, Orlando |
author_facet | de Alencar Junior, Hagamenon Paiotti, Ana Paula Ribeiro de Araújo Filho, Humberto Bezerra Oshima, Celina Tizuko Fujiyama Miszputen, Sender Jankiel Ambrogini‐Júnior, Orlando |
author_sort | de Alencar Junior, Hagamenon |
collection | PubMed |
description | BACKGROUND AND AIM: Human gut microbiota play an important role in metabolism and host physiology. Perturbations of the gut microbial communities lead to the development of various diseases such as inflammatory bowel disease, celiac disease, allergic diseases, and metabolic diseases. Crohn's disease is a chronic inflammatory bowel disease characterized by periods of remission and relapse. Several studies suggest that intestinal inflammation arises due to an abnormal response of the intestinal immune system to the fecal microbiota. The goal of the study was to evaluate the relative amount of four bacterial groups in fecal samples of Crohn's disease patients and their relation to the inflammatory activity. METHODS: We studied stool samples of 105 individuals, 54 with Crohn's disease and 51 as a control group. The DNA extracted from the stool samples was subjected to real‐time polymerase chain reaction (qPCR) for quantification of the Bacteroidetes phylum, class Bacilli, and Bifidobacteriaceae and Enterobacteriaceae families. RESULTS: We found a significant increase in Bacteroidetes in Crohn's disease samples when compared to the control group (14 650 and 2060 CFU/ng DNA, respectively) (P = 0.014). On the other hand, we observed a significant reduction in Bacilli and Bifidobacteriaceae (13 and 58 CFU/ng DNA, respectively) (P < 0.0001). In contrast, patients without any drug treatment presented an increase of Bacilli and Bifidobacteriaceae (102 521 and 6235 CFU/ng DNA, respectively) (P < 0.0001). CONCLUSION: The commensal bacteria were decreased in fecal samples of participants with Crohn's disease when compared to the control group. There was no relation between the disease location and/or disease activity with the microbiota. |
format | Online Article Text |
id | pubmed-7578322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wiley Publishing Asia Pty Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75783222020-10-23 The relationship between the commensal microbiota levels and Crohn's disease activity de Alencar Junior, Hagamenon Paiotti, Ana Paula Ribeiro de Araújo Filho, Humberto Bezerra Oshima, Celina Tizuko Fujiyama Miszputen, Sender Jankiel Ambrogini‐Júnior, Orlando JGH Open Leading Articles BACKGROUND AND AIM: Human gut microbiota play an important role in metabolism and host physiology. Perturbations of the gut microbial communities lead to the development of various diseases such as inflammatory bowel disease, celiac disease, allergic diseases, and metabolic diseases. Crohn's disease is a chronic inflammatory bowel disease characterized by periods of remission and relapse. Several studies suggest that intestinal inflammation arises due to an abnormal response of the intestinal immune system to the fecal microbiota. The goal of the study was to evaluate the relative amount of four bacterial groups in fecal samples of Crohn's disease patients and their relation to the inflammatory activity. METHODS: We studied stool samples of 105 individuals, 54 with Crohn's disease and 51 as a control group. The DNA extracted from the stool samples was subjected to real‐time polymerase chain reaction (qPCR) for quantification of the Bacteroidetes phylum, class Bacilli, and Bifidobacteriaceae and Enterobacteriaceae families. RESULTS: We found a significant increase in Bacteroidetes in Crohn's disease samples when compared to the control group (14 650 and 2060 CFU/ng DNA, respectively) (P = 0.014). On the other hand, we observed a significant reduction in Bacilli and Bifidobacteriaceae (13 and 58 CFU/ng DNA, respectively) (P < 0.0001). In contrast, patients without any drug treatment presented an increase of Bacilli and Bifidobacteriaceae (102 521 and 6235 CFU/ng DNA, respectively) (P < 0.0001). CONCLUSION: The commensal bacteria were decreased in fecal samples of participants with Crohn's disease when compared to the control group. There was no relation between the disease location and/or disease activity with the microbiota. Wiley Publishing Asia Pty Ltd 2020-05-01 /pmc/articles/PMC7578322/ /pubmed/33102745 http://dx.doi.org/10.1002/jgh3.12338 Text en © 2020 The Authors. JGH Open: An open access journal of gastroenterology and hepatology published by Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Leading Articles de Alencar Junior, Hagamenon Paiotti, Ana Paula Ribeiro de Araújo Filho, Humberto Bezerra Oshima, Celina Tizuko Fujiyama Miszputen, Sender Jankiel Ambrogini‐Júnior, Orlando The relationship between the commensal microbiota levels and Crohn's disease activity |
title | The relationship between the commensal microbiota levels and Crohn's disease activity |
title_full | The relationship between the commensal microbiota levels and Crohn's disease activity |
title_fullStr | The relationship between the commensal microbiota levels and Crohn's disease activity |
title_full_unstemmed | The relationship between the commensal microbiota levels and Crohn's disease activity |
title_short | The relationship between the commensal microbiota levels and Crohn's disease activity |
title_sort | relationship between the commensal microbiota levels and crohn's disease activity |
topic | Leading Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578322/ https://www.ncbi.nlm.nih.gov/pubmed/33102745 http://dx.doi.org/10.1002/jgh3.12338 |
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