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High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival

Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, cause...

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Autores principales: Delisle, Lizenn, Pauletto, Marianna, Vidal-Dupiol, Jeremie, Petton, Bruno, Bargelloni, Luca, Montagnani, Caroline, Pernet, Fabrice, Corporeau, Charlotte, Fleury, Elodie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578350/
https://www.ncbi.nlm.nih.gov/pubmed/32816959
http://dx.doi.org/10.1242/jeb.226233
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author Delisle, Lizenn
Pauletto, Marianna
Vidal-Dupiol, Jeremie
Petton, Bruno
Bargelloni, Luca
Montagnani, Caroline
Pernet, Fabrice
Corporeau, Charlotte
Fleury, Elodie
author_facet Delisle, Lizenn
Pauletto, Marianna
Vidal-Dupiol, Jeremie
Petton, Bruno
Bargelloni, Luca
Montagnani, Caroline
Pernet, Fabrice
Corporeau, Charlotte
Fleury, Elodie
author_sort Delisle, Lizenn
collection PubMed
description Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29°C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29°C. Moreover, viral entry into the host might have been limited at 29°C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host–pathogen interactions.
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spelling pubmed-75783502020-10-27 High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival Delisle, Lizenn Pauletto, Marianna Vidal-Dupiol, Jeremie Petton, Bruno Bargelloni, Luca Montagnani, Caroline Pernet, Fabrice Corporeau, Charlotte Fleury, Elodie J Exp Biol Research Article Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29°C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29°C. Moreover, viral entry into the host might have been limited at 29°C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host–pathogen interactions. The Company of Biologists Ltd 2020-10-15 /pmc/articles/PMC7578350/ /pubmed/32816959 http://dx.doi.org/10.1242/jeb.226233 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Delisle, Lizenn
Pauletto, Marianna
Vidal-Dupiol, Jeremie
Petton, Bruno
Bargelloni, Luca
Montagnani, Caroline
Pernet, Fabrice
Corporeau, Charlotte
Fleury, Elodie
High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title_full High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title_fullStr High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title_full_unstemmed High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title_short High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
title_sort high temperature induces transcriptomic changes in crassostrea gigas that hinder progress of ostreid herpesvirus (oshv-1) and promote survival
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578350/
https://www.ncbi.nlm.nih.gov/pubmed/32816959
http://dx.doi.org/10.1242/jeb.226233
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