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High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival
Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, cause...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578350/ https://www.ncbi.nlm.nih.gov/pubmed/32816959 http://dx.doi.org/10.1242/jeb.226233 |
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author | Delisle, Lizenn Pauletto, Marianna Vidal-Dupiol, Jeremie Petton, Bruno Bargelloni, Luca Montagnani, Caroline Pernet, Fabrice Corporeau, Charlotte Fleury, Elodie |
author_facet | Delisle, Lizenn Pauletto, Marianna Vidal-Dupiol, Jeremie Petton, Bruno Bargelloni, Luca Montagnani, Caroline Pernet, Fabrice Corporeau, Charlotte Fleury, Elodie |
author_sort | Delisle, Lizenn |
collection | PubMed |
description | Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29°C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29°C. Moreover, viral entry into the host might have been limited at 29°C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host–pathogen interactions. |
format | Online Article Text |
id | pubmed-7578350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-75783502020-10-27 High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival Delisle, Lizenn Pauletto, Marianna Vidal-Dupiol, Jeremie Petton, Bruno Bargelloni, Luca Montagnani, Caroline Pernet, Fabrice Corporeau, Charlotte Fleury, Elodie J Exp Biol Research Article Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24°C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21°C (reference) and 29°C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29°C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29°C. Moreover, viral entry into the host might have been limited at 29°C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host–pathogen interactions. The Company of Biologists Ltd 2020-10-15 /pmc/articles/PMC7578350/ /pubmed/32816959 http://dx.doi.org/10.1242/jeb.226233 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Delisle, Lizenn Pauletto, Marianna Vidal-Dupiol, Jeremie Petton, Bruno Bargelloni, Luca Montagnani, Caroline Pernet, Fabrice Corporeau, Charlotte Fleury, Elodie High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title | High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title_full | High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title_fullStr | High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title_full_unstemmed | High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title_short | High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival |
title_sort | high temperature induces transcriptomic changes in crassostrea gigas that hinder progress of ostreid herpesvirus (oshv-1) and promote survival |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578350/ https://www.ncbi.nlm.nih.gov/pubmed/32816959 http://dx.doi.org/10.1242/jeb.226233 |
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