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In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp
Interest in periocular (eyelid and eyelashes margins) hygiene has attracted attention recently and a growing number of commercials eye cleanser and shampoos have been marketed. In the present study, a particular eye cleanser foam, Belcils® has been tested against trophozoites and cysts on the facult...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578530/ https://www.ncbi.nlm.nih.gov/pubmed/33099237 http://dx.doi.org/10.1016/j.ijpddr.2020.10.002 |
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author | Sifaoui, Ines Rodríguez-Talavera, Iván Reyes-Batlle, María Rodríguez-Expósito, Rubén L. Rocha-Cabrera, Pedro Piñero, José E. Lorenzo-Morales, Jacob |
author_facet | Sifaoui, Ines Rodríguez-Talavera, Iván Reyes-Batlle, María Rodríguez-Expósito, Rubén L. Rocha-Cabrera, Pedro Piñero, José E. Lorenzo-Morales, Jacob |
author_sort | Sifaoui, Ines |
collection | PubMed |
description | Interest in periocular (eyelid and eyelashes margins) hygiene has attracted attention recently and a growing number of commercials eye cleanser and shampoos have been marketed. In the present study, a particular eye cleanser foam, Belcils® has been tested against trophozoites and cysts on the facultative pathogen Acanthamoeba. Viability was tested by the alamarBlue™ method and the foam was tested for the induction of programmed cell death in order to explore its mode of action. We found that a 1% solution of the foam eliminated both trophozoite and cyst stage of Acanthamoeba spp. After 90 min of incubation, Belcils® induced, DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba. We conclude that the foam destroys the cells by the induction of an apoptosis-like process. The current eye cleanser could be used as part of AK therapy protocol and as prevention from AK infections for contact lens users and post-ocular trauma patients. |
format | Online Article Text |
id | pubmed-7578530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75785302020-10-23 In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp Sifaoui, Ines Rodríguez-Talavera, Iván Reyes-Batlle, María Rodríguez-Expósito, Rubén L. Rocha-Cabrera, Pedro Piñero, José E. Lorenzo-Morales, Jacob Int J Parasitol Drugs Drug Resist Article Interest in periocular (eyelid and eyelashes margins) hygiene has attracted attention recently and a growing number of commercials eye cleanser and shampoos have been marketed. In the present study, a particular eye cleanser foam, Belcils® has been tested against trophozoites and cysts on the facultative pathogen Acanthamoeba. Viability was tested by the alamarBlue™ method and the foam was tested for the induction of programmed cell death in order to explore its mode of action. We found that a 1% solution of the foam eliminated both trophozoite and cyst stage of Acanthamoeba spp. After 90 min of incubation, Belcils® induced, DNA condensation, collapse in the mitochondrial membrane potential and reduction of the ATP level production in Acanthamoeba. We conclude that the foam destroys the cells by the induction of an apoptosis-like process. The current eye cleanser could be used as part of AK therapy protocol and as prevention from AK infections for contact lens users and post-ocular trauma patients. Elsevier 2020-10-14 /pmc/articles/PMC7578530/ /pubmed/33099237 http://dx.doi.org/10.1016/j.ijpddr.2020.10.002 Text en © 2020 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Sifaoui, Ines Rodríguez-Talavera, Iván Reyes-Batlle, María Rodríguez-Expósito, Rubén L. Rocha-Cabrera, Pedro Piñero, José E. Lorenzo-Morales, Jacob In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title | In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title_full | In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title_fullStr | In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title_full_unstemmed | In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title_short | In vitro evaluation of commercial foam Belcils® on Acanthamoeba spp |
title_sort | in vitro evaluation of commercial foam belcils® on acanthamoeba spp |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578530/ https://www.ncbi.nlm.nih.gov/pubmed/33099237 http://dx.doi.org/10.1016/j.ijpddr.2020.10.002 |
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