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IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis

BACKGROUND: Schistosoma haematobium, the helminth causing urogenital schistosomiasis, is a known bladder carcinogen. Despite the causal link between S. haematobium and bladder cancer, the underlying mechanisms are poorly understood. S. haematobium oviposition in the bladder is associated with angiog...

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Autores principales: Mbanefo, Evaristus C., Agbo, Chinwike Terry, Zhao, Yuanlong, Lamanna, Olivia K., Thai, Kim H., Karinshak, Shannon E., Khan, Mohammad Afzal, Fu, Chi-Ling, Odegaard, Justin I., Saltikova, Irina V., Smout, Michael J., Pennington, Luke F., Nicolls, Mark R., Jardetzky, Theodore S., Loukas, Alex, Brindley, Paul J., Falcone, Franco H., Hsieh, Michael H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578584/
https://www.ncbi.nlm.nih.gov/pubmed/33101456
http://dx.doi.org/10.1186/s13027-020-00331-6
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author Mbanefo, Evaristus C.
Agbo, Chinwike Terry
Zhao, Yuanlong
Lamanna, Olivia K.
Thai, Kim H.
Karinshak, Shannon E.
Khan, Mohammad Afzal
Fu, Chi-Ling
Odegaard, Justin I.
Saltikova, Irina V.
Smout, Michael J.
Pennington, Luke F.
Nicolls, Mark R.
Jardetzky, Theodore S.
Loukas, Alex
Brindley, Paul J.
Falcone, Franco H.
Hsieh, Michael H.
author_facet Mbanefo, Evaristus C.
Agbo, Chinwike Terry
Zhao, Yuanlong
Lamanna, Olivia K.
Thai, Kim H.
Karinshak, Shannon E.
Khan, Mohammad Afzal
Fu, Chi-Ling
Odegaard, Justin I.
Saltikova, Irina V.
Smout, Michael J.
Pennington, Luke F.
Nicolls, Mark R.
Jardetzky, Theodore S.
Loukas, Alex
Brindley, Paul J.
Falcone, Franco H.
Hsieh, Michael H.
author_sort Mbanefo, Evaristus C.
collection PubMed
description BACKGROUND: Schistosoma haematobium, the helminth causing urogenital schistosomiasis, is a known bladder carcinogen. Despite the causal link between S. haematobium and bladder cancer, the underlying mechanisms are poorly understood. S. haematobium oviposition in the bladder is associated with angiogenesis and urothelial hyperplasia. These changes may be pre-carcinogenic events in the bladder. We hypothesized that the Interleukin-4-inducing principle of Schistosoma mansoni eggs (IPSE), an S. haematobium egg-secreted “infiltrin” protein that enters host cell nuclei to alter cellular activity, is sufficient to induce angiogenesis and urothelial hyperplasia. Methods: Mouse bladders injected with S. haematobium eggs were analyzed via microscopy for angiogenesis and urothelial hyperplasia. Endothelial and urothelial cell lines were incubated with recombinant IPSE protein or an IPSE mutant protein that lacks the native nuclear localization sequence (NLS-) and proliferation measured using CFSE staining and real-time monitoring of cell growth. IPSE’s effects on urothelial cell cycle status was assayed through propidium iodide staining. Endothelial and urothelial cell uptake of fluorophore-labeled IPSE was measured. Findings: Injection of S. haematobium eggs into the bladder triggers angiogenesis, enhances leakiness of bladder blood vessels, and drives urothelial hyperplasia. Wild type IPSE, but not NLS-, increases proliferation of endothelial and urothelial cells and skews urothelial cells towards S phase. Finally, IPSE is internalized by both endothelial and urothelial cells. Interpretation: IPSE drives endothelial and urothelial proliferation, which may depend on internalization of the molecule. The urothelial effects of IPSE depend upon its NLS. Thus, IPSE is a candidate pro-carcinogenic molecule of S. haematobium. SUMMARY: Schistosoma haematobium acts as a bladder carcinogen through unclear mechanisms. The S. haematobium homolog of IPSE, a secreted schistosome egg immunomodulatory molecule, enhances angiogenesis and urothelial proliferation, hallmarks of pre-carcinogenesis, suggesting IPSE is a key pro-oncogenic molecule of S. haematobium.
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spelling pubmed-75785842020-10-22 IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis Mbanefo, Evaristus C. Agbo, Chinwike Terry Zhao, Yuanlong Lamanna, Olivia K. Thai, Kim H. Karinshak, Shannon E. Khan, Mohammad Afzal Fu, Chi-Ling Odegaard, Justin I. Saltikova, Irina V. Smout, Michael J. Pennington, Luke F. Nicolls, Mark R. Jardetzky, Theodore S. Loukas, Alex Brindley, Paul J. Falcone, Franco H. Hsieh, Michael H. Infect Agent Cancer Research Article BACKGROUND: Schistosoma haematobium, the helminth causing urogenital schistosomiasis, is a known bladder carcinogen. Despite the causal link between S. haematobium and bladder cancer, the underlying mechanisms are poorly understood. S. haematobium oviposition in the bladder is associated with angiogenesis and urothelial hyperplasia. These changes may be pre-carcinogenic events in the bladder. We hypothesized that the Interleukin-4-inducing principle of Schistosoma mansoni eggs (IPSE), an S. haematobium egg-secreted “infiltrin” protein that enters host cell nuclei to alter cellular activity, is sufficient to induce angiogenesis and urothelial hyperplasia. Methods: Mouse bladders injected with S. haematobium eggs were analyzed via microscopy for angiogenesis and urothelial hyperplasia. Endothelial and urothelial cell lines were incubated with recombinant IPSE protein or an IPSE mutant protein that lacks the native nuclear localization sequence (NLS-) and proliferation measured using CFSE staining and real-time monitoring of cell growth. IPSE’s effects on urothelial cell cycle status was assayed through propidium iodide staining. Endothelial and urothelial cell uptake of fluorophore-labeled IPSE was measured. Findings: Injection of S. haematobium eggs into the bladder triggers angiogenesis, enhances leakiness of bladder blood vessels, and drives urothelial hyperplasia. Wild type IPSE, but not NLS-, increases proliferation of endothelial and urothelial cells and skews urothelial cells towards S phase. Finally, IPSE is internalized by both endothelial and urothelial cells. Interpretation: IPSE drives endothelial and urothelial proliferation, which may depend on internalization of the molecule. The urothelial effects of IPSE depend upon its NLS. Thus, IPSE is a candidate pro-carcinogenic molecule of S. haematobium. SUMMARY: Schistosoma haematobium acts as a bladder carcinogen through unclear mechanisms. The S. haematobium homolog of IPSE, a secreted schistosome egg immunomodulatory molecule, enhances angiogenesis and urothelial proliferation, hallmarks of pre-carcinogenesis, suggesting IPSE is a key pro-oncogenic molecule of S. haematobium. BioMed Central 2020-10-22 /pmc/articles/PMC7578584/ /pubmed/33101456 http://dx.doi.org/10.1186/s13027-020-00331-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Mbanefo, Evaristus C.
Agbo, Chinwike Terry
Zhao, Yuanlong
Lamanna, Olivia K.
Thai, Kim H.
Karinshak, Shannon E.
Khan, Mohammad Afzal
Fu, Chi-Ling
Odegaard, Justin I.
Saltikova, Irina V.
Smout, Michael J.
Pennington, Luke F.
Nicolls, Mark R.
Jardetzky, Theodore S.
Loukas, Alex
Brindley, Paul J.
Falcone, Franco H.
Hsieh, Michael H.
IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title_full IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title_fullStr IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title_full_unstemmed IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title_short IPSE, an abundant egg-secreted protein of the carcinogenic helminth Schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
title_sort ipse, an abundant egg-secreted protein of the carcinogenic helminth schistosoma haematobium, promotes proliferation of bladder cancer cells and angiogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578584/
https://www.ncbi.nlm.nih.gov/pubmed/33101456
http://dx.doi.org/10.1186/s13027-020-00331-6
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