A fast impedance-based antimicrobial susceptibility test

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated...

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Autores principales: Spencer, Daniel C., Paton, Teagan F., Mulroney, Kieran T., Inglis, Timothy J. J., Sutton, J. Mark, Morgan, Hywel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578651/
https://www.ncbi.nlm.nih.gov/pubmed/33087704
http://dx.doi.org/10.1038/s41467-020-18902-x
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author Spencer, Daniel C.
Paton, Teagan F.
Mulroney, Kieran T.
Inglis, Timothy J. J.
Sutton, J. Mark
Morgan, Hywel
author_facet Spencer, Daniel C.
Paton, Teagan F.
Mulroney, Kieran T.
Inglis, Timothy J. J.
Sutton, J. Mark
Morgan, Hywel
author_sort Spencer, Daniel C.
collection PubMed
description There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10(5) cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.
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spelling pubmed-75786512020-10-29 A fast impedance-based antimicrobial susceptibility test Spencer, Daniel C. Paton, Teagan F. Mulroney, Kieran T. Inglis, Timothy J. J. Sutton, J. Mark Morgan, Hywel Nat Commun Article There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10(5) cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species. Nature Publishing Group UK 2020-10-21 /pmc/articles/PMC7578651/ /pubmed/33087704 http://dx.doi.org/10.1038/s41467-020-18902-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Spencer, Daniel C.
Paton, Teagan F.
Mulroney, Kieran T.
Inglis, Timothy J. J.
Sutton, J. Mark
Morgan, Hywel
A fast impedance-based antimicrobial susceptibility test
title A fast impedance-based antimicrobial susceptibility test
title_full A fast impedance-based antimicrobial susceptibility test
title_fullStr A fast impedance-based antimicrobial susceptibility test
title_full_unstemmed A fast impedance-based antimicrobial susceptibility test
title_short A fast impedance-based antimicrobial susceptibility test
title_sort fast impedance-based antimicrobial susceptibility test
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578651/
https://www.ncbi.nlm.nih.gov/pubmed/33087704
http://dx.doi.org/10.1038/s41467-020-18902-x
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