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Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions

The tools used to study biofilms generally involve either destructive, end-point analyses or periodic measurements. The advent of the internet of things (IoT) era allows circumvention of these limitations. Here we introduce and detail the development of the BioSpec; a modular, nondestructive, real-t...

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Autores principales: Klopper, Kyle B., de Witt, Riaan N., Bester, Elanna, Dicks, Leon M. T., Wolfaardt, Gideon M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578832/
https://www.ncbi.nlm.nih.gov/pubmed/33087727
http://dx.doi.org/10.1038/s41522-020-00153-9
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author Klopper, Kyle B.
de Witt, Riaan N.
Bester, Elanna
Dicks, Leon M. T.
Wolfaardt, Gideon M.
author_facet Klopper, Kyle B.
de Witt, Riaan N.
Bester, Elanna
Dicks, Leon M. T.
Wolfaardt, Gideon M.
author_sort Klopper, Kyle B.
collection PubMed
description The tools used to study biofilms generally involve either destructive, end-point analyses or periodic measurements. The advent of the internet of things (IoT) era allows circumvention of these limitations. Here we introduce and detail the development of the BioSpec; a modular, nondestructive, real-time monitoring system, which accurately and reliably track changes in biofilm biomass over time. The performance of the system was validated using a commercial spectrophotometer and produced comparable results for variations in planktonic and sessile biomass. BioSpec was combined with the previously developed carbon dioxide evolution measurement system (CEMS) to allow simultaneous measurement of biofilm biomass and metabolic activity and revealed a differential response of these interrelated parameters to changing environmental conditions. The application of this system can facilitate a greater understanding of biofilm mass–function relationships and aid in the development of biofilm control strategies.
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spelling pubmed-75788322020-10-23 Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions Klopper, Kyle B. de Witt, Riaan N. Bester, Elanna Dicks, Leon M. T. Wolfaardt, Gideon M. NPJ Biofilms Microbiomes Article The tools used to study biofilms generally involve either destructive, end-point analyses or periodic measurements. The advent of the internet of things (IoT) era allows circumvention of these limitations. Here we introduce and detail the development of the BioSpec; a modular, nondestructive, real-time monitoring system, which accurately and reliably track changes in biofilm biomass over time. The performance of the system was validated using a commercial spectrophotometer and produced comparable results for variations in planktonic and sessile biomass. BioSpec was combined with the previously developed carbon dioxide evolution measurement system (CEMS) to allow simultaneous measurement of biofilm biomass and metabolic activity and revealed a differential response of these interrelated parameters to changing environmental conditions. The application of this system can facilitate a greater understanding of biofilm mass–function relationships and aid in the development of biofilm control strategies. Nature Publishing Group UK 2020-10-21 /pmc/articles/PMC7578832/ /pubmed/33087727 http://dx.doi.org/10.1038/s41522-020-00153-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Klopper, Kyle B.
de Witt, Riaan N.
Bester, Elanna
Dicks, Leon M. T.
Wolfaardt, Gideon M.
Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title_full Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title_fullStr Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title_full_unstemmed Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title_short Biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
title_sort biofilm dynamics: linking in situ biofilm biomass and metabolic activity measurements in real-time under continuous flow conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578832/
https://www.ncbi.nlm.nih.gov/pubmed/33087727
http://dx.doi.org/10.1038/s41522-020-00153-9
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