Cargando…

LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability

The role of the LMNA gene in the development and progression of hepatocellular carcinoma (HCC) and the associated molecular mechanism is not yet clear. Therefore, the purpose of this study was to evaluate the relationship between LMNA and HCC. LMNA gene expression in normal tissues and corresponding...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Heng, Li, Dongming, Zhou, Ling, Kan, Shuang, He, Guozhang, Zhou, Kun, Wang, Liping, Chen, Ming, Shu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578910/
https://www.ncbi.nlm.nih.gov/pubmed/32896989
http://dx.doi.org/10.1111/jcmm.15829
_version_ 1783598467943235584
author Liu, Heng
Li, Dongming
Zhou, Ling
Kan, Shuang
He, Guozhang
Zhou, Kun
Wang, Liping
Chen, Ming
Shu, Wei
author_facet Liu, Heng
Li, Dongming
Zhou, Ling
Kan, Shuang
He, Guozhang
Zhou, Kun
Wang, Liping
Chen, Ming
Shu, Wei
author_sort Liu, Heng
collection PubMed
description The role of the LMNA gene in the development and progression of hepatocellular carcinoma (HCC) and the associated molecular mechanism is not yet clear. Therefore, the purpose of this study was to evaluate the relationship between LMNA and HCC. LMNA gene expression in normal tissues and corresponding tumours was evaluated and the Kaplan–Meier survival analysis was performed. Next, the LMNA gene was knocked out in the 293T and HepG2 cell lines using the CRISPR/Cas9 technique. Subsequently, the proliferation, migration and colony formation rate of the two LMNA knockout cell lines were analysed. Finally, the molecular mechanism affecting the tumorigenesis due to the loss of the LMNA gene was evaluated. The results showed that the LMNA gene was abnormally expressed in many tumours, and the survival rate of the HCC patients with a high expression of the LMNA gene was significantly reduced compared with the rate in patients with a low LMNA expression. The knockout of the LMNA gene in the HCC cell line HepG2 resulted in a decreased tumorigenicity, up‐regulation of the P16 expression and down‐regulation of the CDK1 expression. These findings suggested that LMNA might function as an oncogene in HCC and provided a potential new target for the diagnosis and treatment of HCC.
format Online
Article
Text
id pubmed-7578910
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-75789102020-10-23 LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability Liu, Heng Li, Dongming Zhou, Ling Kan, Shuang He, Guozhang Zhou, Kun Wang, Liping Chen, Ming Shu, Wei J Cell Mol Med Original Articles The role of the LMNA gene in the development and progression of hepatocellular carcinoma (HCC) and the associated molecular mechanism is not yet clear. Therefore, the purpose of this study was to evaluate the relationship between LMNA and HCC. LMNA gene expression in normal tissues and corresponding tumours was evaluated and the Kaplan–Meier survival analysis was performed. Next, the LMNA gene was knocked out in the 293T and HepG2 cell lines using the CRISPR/Cas9 technique. Subsequently, the proliferation, migration and colony formation rate of the two LMNA knockout cell lines were analysed. Finally, the molecular mechanism affecting the tumorigenesis due to the loss of the LMNA gene was evaluated. The results showed that the LMNA gene was abnormally expressed in many tumours, and the survival rate of the HCC patients with a high expression of the LMNA gene was significantly reduced compared with the rate in patients with a low LMNA expression. The knockout of the LMNA gene in the HCC cell line HepG2 resulted in a decreased tumorigenicity, up‐regulation of the P16 expression and down‐regulation of the CDK1 expression. These findings suggested that LMNA might function as an oncogene in HCC and provided a potential new target for the diagnosis and treatment of HCC. John Wiley and Sons Inc. 2020-09-08 2020-10 /pmc/articles/PMC7578910/ /pubmed/32896989 http://dx.doi.org/10.1111/jcmm.15829 Text en © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liu, Heng
Li, Dongming
Zhou, Ling
Kan, Shuang
He, Guozhang
Zhou, Kun
Wang, Liping
Chen, Ming
Shu, Wei
LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title_full LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title_fullStr LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title_full_unstemmed LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title_short LMNA functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
title_sort lmna functions as an oncogene in hepatocellular carcinoma by regulating the proliferation and migration ability
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7578910/
https://www.ncbi.nlm.nih.gov/pubmed/32896989
http://dx.doi.org/10.1111/jcmm.15829
work_keys_str_mv AT liuheng lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT lidongming lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT zhouling lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT kanshuang lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT heguozhang lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT zhoukun lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT wangliping lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT chenming lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability
AT shuwei lmnafunctionsasanoncogeneinhepatocellularcarcinomabyregulatingtheproliferationandmigrationability