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Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy
BACKGROUND: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical app...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579254/ https://www.ncbi.nlm.nih.gov/pubmed/30813881 http://dx.doi.org/10.2174/1574884714666190226093119 |
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author | Yulug, Burak Ozansoy, Mehmet Alokten, Merve Ozansoy, Muzaffer B.C. Cankaya, Seyda Hanoglu, Lutfu Kilic, Ulkan Kilic, Ertugrul |
author_facet | Yulug, Burak Ozansoy, Mehmet Alokten, Merve Ozansoy, Muzaffer B.C. Cankaya, Seyda Hanoglu, Lutfu Kilic, Ulkan Kilic, Ertugrul |
author_sort | Yulug, Burak |
collection | PubMed |
description | BACKGROUND: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical application of such candidate molecules when it comes to human patients. METHODS: After 24 hours of culturing, three different minocycline (Sigma-Aldrich, M9511, Germany) concentrations (1 µM, 10 µM and 100 µM) were added to the primary cortical neurons 15 minutes before laser axotomy procedure in order to observe protective effect of minocycline in these dosages. RESULTS: Here, we have shown that minocycline exerted a significant neuroprotective effect at 1 and 100μM doses. Beyond confirming the neuroprotective effect of minocycline in a more standardised and advanced in-vitro trauma model, our findings could have important implications for future studies that concentrate on the translational block between animal and human studies. CONCLUSION: Such sophisticated approaches might also help to conquer the influence of human-made variabilities in critical experimental injury models. To the best of our knowledge, this is the first study showing that minocycline increases in-vitro neuronal cell survival after laser-axotomy. |
format | Online Article Text |
id | pubmed-7579254 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Bentham Science Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-75792542020-10-28 Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy Yulug, Burak Ozansoy, Mehmet Alokten, Merve Ozansoy, Muzaffer B.C. Cankaya, Seyda Hanoglu, Lutfu Kilic, Ulkan Kilic, Ertugrul Curr Clin Pharmacol Article BACKGROUND: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical application of such candidate molecules when it comes to human patients. METHODS: After 24 hours of culturing, three different minocycline (Sigma-Aldrich, M9511, Germany) concentrations (1 µM, 10 µM and 100 µM) were added to the primary cortical neurons 15 minutes before laser axotomy procedure in order to observe protective effect of minocycline in these dosages. RESULTS: Here, we have shown that minocycline exerted a significant neuroprotective effect at 1 and 100μM doses. Beyond confirming the neuroprotective effect of minocycline in a more standardised and advanced in-vitro trauma model, our findings could have important implications for future studies that concentrate on the translational block between animal and human studies. CONCLUSION: Such sophisticated approaches might also help to conquer the influence of human-made variabilities in critical experimental injury models. To the best of our knowledge, this is the first study showing that minocycline increases in-vitro neuronal cell survival after laser-axotomy. Bentham Science Publishers 2020-08 2020-08 /pmc/articles/PMC7579254/ /pubmed/30813881 http://dx.doi.org/10.2174/1574884714666190226093119 Text en © 2020 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Article Yulug, Burak Ozansoy, Mehmet Alokten, Merve Ozansoy, Muzaffer B.C. Cankaya, Seyda Hanoglu, Lutfu Kilic, Ulkan Kilic, Ertugrul Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title | Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title_full | Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title_fullStr | Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title_full_unstemmed | Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title_short | Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy |
title_sort | minocycline increases in-vitro cortical neuronal cell survival after laser induced axotomy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579254/ https://www.ncbi.nlm.nih.gov/pubmed/30813881 http://dx.doi.org/10.2174/1574884714666190226093119 |
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