Identification of a Novel Pathogen Using Family-Wide PCR: Initial Confirmation of COVID-19 in Thailand

In resource-limited countries, early detection of novel pathogens is often challenging, due to financial and technical constraints. This study reports the efficacy of family-wide polymerase chain reaction (PCR) in screening, detecting, and identifying initial cases of the novel SARS-CoV-2 in Thailan...

Descripción completa

Detalles Bibliográficos
Autores principales: Wacharapluesadee, Supaporn, Buathong, Rome, Iamsirithawon, Sopon, Chaifoo, Walairat, Ponpinit, Teerada, Ruchisrisarod, Chanida, Sonpee, Chanikarn, Katasrila, Panticha, Yomrat, Siriporn, Ghai, Siriporn, Sirivichayakul, Sunee, Okada, Pilailuk, Mekha, Nanthawan, Karnkawinpong, Opart, Uttayamakul, Sumonmal, Vachiraphan, Apichart, Plipat, Tanarak, Hemachudha, Thiravat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Public Health
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579402/
https://www.ncbi.nlm.nih.gov/pubmed/33134237
http://dx.doi.org/10.3389/fpubh.2020.555013
Descripción
Sumario:In resource-limited countries, early detection of novel pathogens is often challenging, due to financial and technical constraints. This study reports the efficacy of family-wide polymerase chain reaction (PCR) in screening, detecting, and identifying initial cases of the novel SARS-CoV-2 in Thailand. Respiratory secretions were collected from suspected individuals traveling from Wuhan, China to Thailand at the beginning of January 2020. Family-wide PCR assays yielded positive results for coronavirus in one traveler within 12 h on January 8, 2020. Nucleotide sequences (290 bp) showed 100% similarity to SARS-CoV-2. The whole genome sequence was further characterized by Next Generation Sequencing (NGS) for confirmation. Combining family-wide PCR, as a rapid screening tool, with NGS, for full genome characterization, could facilitate early detection and confirmation of a novel pathogen and enable early containment of a disease outbreak.

Ejemplares similares