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ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21
BACKGROUND: The upregulation of ADAM17 has been reported to be associated with invasion and metastasis in various tumors, however the molecular mechanism of ADAM17 in the progression of hepatocellular carcinoma (HCC) remain to be clarified. Human matrix metalloproteinase 21 (MMP21), the newest membe...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579888/ https://www.ncbi.nlm.nih.gov/pubmed/33100908 http://dx.doi.org/10.1186/s12935-020-01556-6 |
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author | Xiang, Yuqi Liu, Liyu Wang, Ying Li, Bo Peng, Jinwu Feng, Deyun |
author_facet | Xiang, Yuqi Liu, Liyu Wang, Ying Li, Bo Peng, Jinwu Feng, Deyun |
author_sort | Xiang, Yuqi |
collection | PubMed |
description | BACKGROUND: The upregulation of ADAM17 has been reported to be associated with invasion and metastasis in various tumors, however the molecular mechanism of ADAM17 in the progression of hepatocellular carcinoma (HCC) remain to be clarified. Human matrix metalloproteinase 21 (MMP21), the newest member of the MMP gene family, has been suggested to play an important role in embryogenesis and tumor progression. So far, nothing is known about the relationship between ADAM17 and MMP21. METHODS: In this study, the expression level of ADAM17 and MMP21 in HCC tissues was measured by immunohistochemistry. The Scratch wounding assay and Transwell were used to identify the invasion and metastasis ability. ELISA was used to evaluate the production of MMP21. Coimmunoprecipitation experiments demonstrated a direct association between ADAM17 and MMP21. HPLC was used to confirmed that ADAM17 participated in the maturation of MMP21. RESULTS: Our present data indicated that ADAM17 and MMP21 was significantly upregulated in human HCC tissues. Knockdown of ADAM17 in HCC inhibited cell invasion and metastasis. Moreover, ADAM17 regulates the secretion and expression of MMP21. Furthermore we discovered a direct association between ADAM17 and MMP21, and we also found MMP21 prodomain could be cleaved by ADAM17. CONCLUSION: Our data suggest that ADAM17 plays an important role in the development of HCC invasion and metastasis and this function may be implement by MMP21. |
format | Online Article Text |
id | pubmed-7579888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75798882020-10-22 ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 Xiang, Yuqi Liu, Liyu Wang, Ying Li, Bo Peng, Jinwu Feng, Deyun Cancer Cell Int Primary Research BACKGROUND: The upregulation of ADAM17 has been reported to be associated with invasion and metastasis in various tumors, however the molecular mechanism of ADAM17 in the progression of hepatocellular carcinoma (HCC) remain to be clarified. Human matrix metalloproteinase 21 (MMP21), the newest member of the MMP gene family, has been suggested to play an important role in embryogenesis and tumor progression. So far, nothing is known about the relationship between ADAM17 and MMP21. METHODS: In this study, the expression level of ADAM17 and MMP21 in HCC tissues was measured by immunohistochemistry. The Scratch wounding assay and Transwell were used to identify the invasion and metastasis ability. ELISA was used to evaluate the production of MMP21. Coimmunoprecipitation experiments demonstrated a direct association between ADAM17 and MMP21. HPLC was used to confirmed that ADAM17 participated in the maturation of MMP21. RESULTS: Our present data indicated that ADAM17 and MMP21 was significantly upregulated in human HCC tissues. Knockdown of ADAM17 in HCC inhibited cell invasion and metastasis. Moreover, ADAM17 regulates the secretion and expression of MMP21. Furthermore we discovered a direct association between ADAM17 and MMP21, and we also found MMP21 prodomain could be cleaved by ADAM17. CONCLUSION: Our data suggest that ADAM17 plays an important role in the development of HCC invasion and metastasis and this function may be implement by MMP21. BioMed Central 2020-10-21 /pmc/articles/PMC7579888/ /pubmed/33100908 http://dx.doi.org/10.1186/s12935-020-01556-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Xiang, Yuqi Liu, Liyu Wang, Ying Li, Bo Peng, Jinwu Feng, Deyun ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title | ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title_full | ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title_fullStr | ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title_full_unstemmed | ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title_short | ADAM17 promotes the invasion of hepatocellular carcinoma via upregulation MMP21 |
title_sort | adam17 promotes the invasion of hepatocellular carcinoma via upregulation mmp21 |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579888/ https://www.ncbi.nlm.nih.gov/pubmed/33100908 http://dx.doi.org/10.1186/s12935-020-01556-6 |
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