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In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts

OBJECTIVES: Cigarette smoke aqueous aerosol extracts (AqE) have been used for assessing tobacco products, particularly with in vitro models such as oxidative stress and inflammation. These test articles can be generated easily, but there are no standardised methods for the generation and characteris...

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Autores principales: Taylor, Mark, Santopietro, Simone, Baxter, Andrew, East, Nicole, Breheny, Damien, Thorne, David, Gaça, Marianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579917/
https://www.ncbi.nlm.nih.gov/pubmed/33087173
http://dx.doi.org/10.1186/s13104-020-05337-2
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author Taylor, Mark
Santopietro, Simone
Baxter, Andrew
East, Nicole
Breheny, Damien
Thorne, David
Gaça, Marianna
author_facet Taylor, Mark
Santopietro, Simone
Baxter, Andrew
East, Nicole
Breheny, Damien
Thorne, David
Gaça, Marianna
author_sort Taylor, Mark
collection PubMed
description OBJECTIVES: Cigarette smoke aqueous aerosol extracts (AqE) have been used for assessing tobacco products, particularly with in vitro models such as oxidative stress and inflammation. These test articles can be generated easily, but there are no standardised methods for the generation and characterisation or stability. We investigated the effects of pro-oxidant smoke-derived chemicals by using 3R4F AqE generated under standardised conditioning and smoking regimes and assessed the stability over 31-week timeframe. Twenty batches generated from ten puffs per cigarette bubbled through 20 ml cell culture media were used fresh and thawed from frozen aliquots stored at – 80 ºC. RESULTS: Nicotine levels quantified by gas chromatography/mass spectrometry and optical density at 260 nm showed chemical and physical stability from week 0 (fresh sample) to weeks 1, 4, 8 and 31 (frozen samples). No significant change in H292 human bronchial epithelial cell viability or oxidative stress were observed between fresh AqE at week 0 and frozen AqE at 31 weeks. AqEs generated by our protocol were stable for up to 31 weeks for all tested end points, suggesting that it may not be necessary to use freshly generated AqE for each study, thus reducing batch-to-batch variability.
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spelling pubmed-75799172020-10-22 In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts Taylor, Mark Santopietro, Simone Baxter, Andrew East, Nicole Breheny, Damien Thorne, David Gaça, Marianna BMC Res Notes Research Note OBJECTIVES: Cigarette smoke aqueous aerosol extracts (AqE) have been used for assessing tobacco products, particularly with in vitro models such as oxidative stress and inflammation. These test articles can be generated easily, but there are no standardised methods for the generation and characterisation or stability. We investigated the effects of pro-oxidant smoke-derived chemicals by using 3R4F AqE generated under standardised conditioning and smoking regimes and assessed the stability over 31-week timeframe. Twenty batches generated from ten puffs per cigarette bubbled through 20 ml cell culture media were used fresh and thawed from frozen aliquots stored at – 80 ºC. RESULTS: Nicotine levels quantified by gas chromatography/mass spectrometry and optical density at 260 nm showed chemical and physical stability from week 0 (fresh sample) to weeks 1, 4, 8 and 31 (frozen samples). No significant change in H292 human bronchial epithelial cell viability or oxidative stress were observed between fresh AqE at week 0 and frozen AqE at 31 weeks. AqEs generated by our protocol were stable for up to 31 weeks for all tested end points, suggesting that it may not be necessary to use freshly generated AqE for each study, thus reducing batch-to-batch variability. BioMed Central 2020-10-21 /pmc/articles/PMC7579917/ /pubmed/33087173 http://dx.doi.org/10.1186/s13104-020-05337-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Taylor, Mark
Santopietro, Simone
Baxter, Andrew
East, Nicole
Breheny, Damien
Thorne, David
Gaça, Marianna
In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title_full In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title_fullStr In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title_full_unstemmed In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title_short In vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
title_sort in vitro biological assessment of the stability of cigarette smoke aqueous aerosol extracts
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579917/
https://www.ncbi.nlm.nih.gov/pubmed/33087173
http://dx.doi.org/10.1186/s13104-020-05337-2
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