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Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells

BACKGROUND: Hydrogen sulfide (H(2)S) is a gas signal molecule involved in regulating plants tolerance to heavy metals stress. In this study, we investigated the role of H(2)S in cadmium-(Cd-) induced cell death of root tips of cucumber seedlings. RESULTS: The results showed that the application of 2...

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Autores principales: Luo, Shilei, Tang, Zhongqi, Yu, Jihua, Liao, Weibiao, Xie, Jianming, Lv, Jian, Feng, Zhi, Dawuda, Mohammed Mujitaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579943/
https://www.ncbi.nlm.nih.gov/pubmed/33087071
http://dx.doi.org/10.1186/s12870-020-02687-8
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author Luo, Shilei
Tang, Zhongqi
Yu, Jihua
Liao, Weibiao
Xie, Jianming
Lv, Jian
Feng, Zhi
Dawuda, Mohammed Mujitaba
author_facet Luo, Shilei
Tang, Zhongqi
Yu, Jihua
Liao, Weibiao
Xie, Jianming
Lv, Jian
Feng, Zhi
Dawuda, Mohammed Mujitaba
author_sort Luo, Shilei
collection PubMed
description BACKGROUND: Hydrogen sulfide (H(2)S) is a gas signal molecule involved in regulating plants tolerance to heavy metals stress. In this study, we investigated the role of H(2)S in cadmium-(Cd-) induced cell death of root tips of cucumber seedlings. RESULTS: The results showed that the application of 200 μM Cd caused cell death, increased the content of reactive oxygen species (ROS), chromatin condensation, the release of Cytochrome c (Cyt c) from mitochondria and activated caspase-3-like protease. Pretreatment of seedlings with 100 μM sodium hydrogen sulfide (NaHS, a H(2)S donor) effectively alleviated the growth inhibition and reduced cell death of root tips caused by Cd stress. Additionally, NaHS + Cd treatment could decrease the ROS level and enhanced antioxidant enzyme activity. Pretreatment with NaHS also inhibited the release of Cyt c from the mitochondria, the opening of the mitochondrial permeability transition pore (MPTP), and the activity of caspase-3-like protease in the root tips of cucumber seedling under Cd stress. CONCLUSION: H(2)S inhibited Cd-induced cell death in cucumber root tips by reducing ROS accumulation, activating the antioxidant system, inhibiting mitochondrial Cyt c release and reducing the opening of the MPTP. The results suggest that H(2)S is a negative regulator of Cd-induced cell death in the root tips of cucumber seedling.
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spelling pubmed-75799432020-10-22 Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells Luo, Shilei Tang, Zhongqi Yu, Jihua Liao, Weibiao Xie, Jianming Lv, Jian Feng, Zhi Dawuda, Mohammed Mujitaba BMC Plant Biol Research Article BACKGROUND: Hydrogen sulfide (H(2)S) is a gas signal molecule involved in regulating plants tolerance to heavy metals stress. In this study, we investigated the role of H(2)S in cadmium-(Cd-) induced cell death of root tips of cucumber seedlings. RESULTS: The results showed that the application of 200 μM Cd caused cell death, increased the content of reactive oxygen species (ROS), chromatin condensation, the release of Cytochrome c (Cyt c) from mitochondria and activated caspase-3-like protease. Pretreatment of seedlings with 100 μM sodium hydrogen sulfide (NaHS, a H(2)S donor) effectively alleviated the growth inhibition and reduced cell death of root tips caused by Cd stress. Additionally, NaHS + Cd treatment could decrease the ROS level and enhanced antioxidant enzyme activity. Pretreatment with NaHS also inhibited the release of Cyt c from the mitochondria, the opening of the mitochondrial permeability transition pore (MPTP), and the activity of caspase-3-like protease in the root tips of cucumber seedling under Cd stress. CONCLUSION: H(2)S inhibited Cd-induced cell death in cucumber root tips by reducing ROS accumulation, activating the antioxidant system, inhibiting mitochondrial Cyt c release and reducing the opening of the MPTP. The results suggest that H(2)S is a negative regulator of Cd-induced cell death in the root tips of cucumber seedling. BioMed Central 2020-10-21 /pmc/articles/PMC7579943/ /pubmed/33087071 http://dx.doi.org/10.1186/s12870-020-02687-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Luo, Shilei
Tang, Zhongqi
Yu, Jihua
Liao, Weibiao
Xie, Jianming
Lv, Jian
Feng, Zhi
Dawuda, Mohammed Mujitaba
Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title_full Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title_fullStr Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title_full_unstemmed Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title_short Hydrogen sulfide negatively regulates cd-induced cell death in cucumber (Cucumis sativus L) root tip cells
title_sort hydrogen sulfide negatively regulates cd-induced cell death in cucumber (cucumis sativus l) root tip cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579943/
https://www.ncbi.nlm.nih.gov/pubmed/33087071
http://dx.doi.org/10.1186/s12870-020-02687-8
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