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Assaying the effect of yeasts on growth of fungi associated with disease

BACKGROUND: Pathogenic fungi often cause serious infections mainly in immunocompromised persons. The number of infections caused by the non-albicans Candida or other species has significantly increased over the last years. These infections present a major challenge in the health sector because these...

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Autores principales: Horváth, Enikő, Sipiczki, Matthias, Csoma, Hajnalka, Miklós, Ida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579944/
https://www.ncbi.nlm.nih.gov/pubmed/33087058
http://dx.doi.org/10.1186/s12866-020-01942-0
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author Horváth, Enikő
Sipiczki, Matthias
Csoma, Hajnalka
Miklós, Ida
author_facet Horváth, Enikő
Sipiczki, Matthias
Csoma, Hajnalka
Miklós, Ida
author_sort Horváth, Enikő
collection PubMed
description BACKGROUND: Pathogenic fungi often cause serious infections mainly in immunocompromised persons. The number of infections caused by the non-albicans Candida or other species has significantly increased over the last years. These infections present a major challenge in the health sector because these pathogenic fungi have strong virulence and often show resistance to the commonly used antifungal treatments. To solve the problems caused by the drug resistant pathogenic fungi, it is necessary to find new antifungal agents and their sources. The aim of this study was to give evidence that yeasts can effectively fight against strains which belong to pathogenic fungi and reveal those yeasts which are able to inhibit growth of Kodamaea ohmeri, Pichia kudriavzevii, Naganishia albida or Candida tropicalis. Furthermore, we wanted to determine the effects of certain culturing factors on the growth inhibition. RESULTS: Our screening revealed that although the strains belonging to pathogenic species were much more tolerant to the yeast-produced bioactive agents than the non-disease-associated yeasts, growth of Kodamaea ohmeri and Candida tropicalis could be inhibited by Metschnikowia andauensis, while Naganishia albida could be controlled by Pichia anomala or Candida tropicalis. Our data proved that the experimental circumstances could have a serious impact on the inhibitory capacity of the yeasts. Appearance of inhibition strongly depended on media, pH and temperature. Our data also shed some light on the fact that Pichia kudriavzevii must have high natural resistance to the yeast-produced agents, while other species, such as Saccharomycopsis crataegensis belonged to the easily inhibitable species. CONCLUSIONS: Our study suggests that yeast-produced bioactive agents could be potential growth inhibitory agents against the disease-associated fungi and yeasts can also contribute to alternative approaches to combat against pathogenic fungi. Our data revealed an important role of the culturing factors in inhibition and pointed to the complex nature of antagonism.
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spelling pubmed-75799442020-10-22 Assaying the effect of yeasts on growth of fungi associated with disease Horváth, Enikő Sipiczki, Matthias Csoma, Hajnalka Miklós, Ida BMC Microbiol Research Article BACKGROUND: Pathogenic fungi often cause serious infections mainly in immunocompromised persons. The number of infections caused by the non-albicans Candida or other species has significantly increased over the last years. These infections present a major challenge in the health sector because these pathogenic fungi have strong virulence and often show resistance to the commonly used antifungal treatments. To solve the problems caused by the drug resistant pathogenic fungi, it is necessary to find new antifungal agents and their sources. The aim of this study was to give evidence that yeasts can effectively fight against strains which belong to pathogenic fungi and reveal those yeasts which are able to inhibit growth of Kodamaea ohmeri, Pichia kudriavzevii, Naganishia albida or Candida tropicalis. Furthermore, we wanted to determine the effects of certain culturing factors on the growth inhibition. RESULTS: Our screening revealed that although the strains belonging to pathogenic species were much more tolerant to the yeast-produced bioactive agents than the non-disease-associated yeasts, growth of Kodamaea ohmeri and Candida tropicalis could be inhibited by Metschnikowia andauensis, while Naganishia albida could be controlled by Pichia anomala or Candida tropicalis. Our data proved that the experimental circumstances could have a serious impact on the inhibitory capacity of the yeasts. Appearance of inhibition strongly depended on media, pH and temperature. Our data also shed some light on the fact that Pichia kudriavzevii must have high natural resistance to the yeast-produced agents, while other species, such as Saccharomycopsis crataegensis belonged to the easily inhibitable species. CONCLUSIONS: Our study suggests that yeast-produced bioactive agents could be potential growth inhibitory agents against the disease-associated fungi and yeasts can also contribute to alternative approaches to combat against pathogenic fungi. Our data revealed an important role of the culturing factors in inhibition and pointed to the complex nature of antagonism. BioMed Central 2020-10-21 /pmc/articles/PMC7579944/ /pubmed/33087058 http://dx.doi.org/10.1186/s12866-020-01942-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Horváth, Enikő
Sipiczki, Matthias
Csoma, Hajnalka
Miklós, Ida
Assaying the effect of yeasts on growth of fungi associated with disease
title Assaying the effect of yeasts on growth of fungi associated with disease
title_full Assaying the effect of yeasts on growth of fungi associated with disease
title_fullStr Assaying the effect of yeasts on growth of fungi associated with disease
title_full_unstemmed Assaying the effect of yeasts on growth of fungi associated with disease
title_short Assaying the effect of yeasts on growth of fungi associated with disease
title_sort assaying the effect of yeasts on growth of fungi associated with disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579944/
https://www.ncbi.nlm.nih.gov/pubmed/33087058
http://dx.doi.org/10.1186/s12866-020-01942-0
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