Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes

The metabolic activity of cells is interrelated with cell signaling, functions, and fate. Uncontrolled cancer cell proliferation requires metabolic adaptations. Research focusing on understanding the characteristics of cell metabolism is crucial for the development of novel diagnostic and therapeuti...

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Detalles Bibliográficos
Autores principales: Rauter, Thomas, Depaoli, Maria R., Bischof, Helmut, Graier, Wolfgang F., Malli, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580088/
https://www.ncbi.nlm.nih.gov/pubmed/33111094
http://dx.doi.org/10.1016/j.xpro.2020.100048
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author Rauter, Thomas
Depaoli, Maria R.
Bischof, Helmut
Graier, Wolfgang F.
Malli, Roland
author_facet Rauter, Thomas
Depaoli, Maria R.
Bischof, Helmut
Graier, Wolfgang F.
Malli, Roland
author_sort Rauter, Thomas
collection PubMed
description The metabolic activity of cells is interrelated with cell signaling, functions, and fate. Uncontrolled cancer cell proliferation requires metabolic adaptations. Research focusing on understanding the characteristics of cell metabolism is crucial for the development of novel diagnostic and therapeutic strategies. Here, we describe protocols for the ATP profiling of single cancer cells by fluorescence live-cell imaging. In response to distinct metabolic inhibitions, we record individual mitochondrial ATP dynamics using established Förster resonance energy transfer-based genetically encoded fluorescent ATP probes. For complete details on the use and execution of this protocol, please refer to Depaoli et al. (2018).
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spelling pubmed-75800882020-10-26 Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes Rauter, Thomas Depaoli, Maria R. Bischof, Helmut Graier, Wolfgang F. Malli, Roland STAR Protoc Protocol The metabolic activity of cells is interrelated with cell signaling, functions, and fate. Uncontrolled cancer cell proliferation requires metabolic adaptations. Research focusing on understanding the characteristics of cell metabolism is crucial for the development of novel diagnostic and therapeutic strategies. Here, we describe protocols for the ATP profiling of single cancer cells by fluorescence live-cell imaging. In response to distinct metabolic inhibitions, we record individual mitochondrial ATP dynamics using established Förster resonance energy transfer-based genetically encoded fluorescent ATP probes. For complete details on the use and execution of this protocol, please refer to Depaoli et al. (2018). Elsevier 2020-06-08 /pmc/articles/PMC7580088/ /pubmed/33111094 http://dx.doi.org/10.1016/j.xpro.2020.100048 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rauter, Thomas
Depaoli, Maria R.
Bischof, Helmut
Graier, Wolfgang F.
Malli, Roland
Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title_full Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title_fullStr Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title_full_unstemmed Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title_short Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes
title_sort metabolic profiling of single cancer cells using mitochondrial atp probes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580088/
https://www.ncbi.nlm.nih.gov/pubmed/33111094
http://dx.doi.org/10.1016/j.xpro.2020.100048
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