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Protocol for Genome Editing to Produce Multiple Mutants in Wheat

Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mut...

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Detalles Bibliográficos
Autores principales:	Abe, Fumitaka, Ishida, Yuji, Hisano, Hiroshi, Endo, Masaki, Komari, Toshihiko, Toki, Seiichi, Sato, Kazuhiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580089/ https://www.ncbi.nlm.nih.gov/pubmed/33111098 http://dx.doi.org/10.1016/j.xpro.2020.100053

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