Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons

Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured...

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Detalles Bibliográficos
Autores principales: Um, Ji Won, Han, Kyung Ah, Choi, Se-Young, Ko, Jaewon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580093/
https://www.ncbi.nlm.nih.gov/pubmed/33111124
http://dx.doi.org/10.1016/j.xpro.2020.100095
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author Um, Ji Won
Han, Kyung Ah
Choi, Se-Young
Ko, Jaewon
author_facet Um, Ji Won
Han, Kyung Ah
Choi, Se-Young
Ko, Jaewon
author_sort Um, Ji Won
collection PubMed
description Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured neurons, expression of synaptic vesicle-enriched proteins, and quantification procedures. Utilizing neurons from postnatal transgenic mice, this method can be applied to investigate the roles of synaptic genes in regulating vesicle dynamics at synaptic sites. For complete details on the use and execution of this protocol, please refer to Han et al. (2020a).
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spelling pubmed-75800932020-10-26 Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons Um, Ji Won Han, Kyung Ah Choi, Se-Young Ko, Jaewon STAR Protoc Protocol Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured neurons, expression of synaptic vesicle-enriched proteins, and quantification procedures. Utilizing neurons from postnatal transgenic mice, this method can be applied to investigate the roles of synaptic genes in regulating vesicle dynamics at synaptic sites. For complete details on the use and execution of this protocol, please refer to Han et al. (2020a). Elsevier 2020-08-21 /pmc/articles/PMC7580093/ /pubmed/33111124 http://dx.doi.org/10.1016/j.xpro.2020.100095 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Um, Ji Won
Han, Kyung Ah
Choi, Se-Young
Ko, Jaewon
Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title_full Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title_fullStr Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title_full_unstemmed Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title_short Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons
title_sort protocol for quantitative analysis of synaptic vesicle clustering in axons of cultured neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580093/
https://www.ncbi.nlm.nih.gov/pubmed/33111124
http://dx.doi.org/10.1016/j.xpro.2020.100095
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