Protocol for Imaging of Mitoflashes in Live Cardiomyocytes

We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration fu...

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Detalles Bibliográficos
Autores principales: Li, Anqi, Qin, Yuan, Gao, Meng, Jiang, Wenting, Liu, Bilin, Tian, Xiangang, Gong, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580095/
https://www.ncbi.nlm.nih.gov/pubmed/33111128
http://dx.doi.org/10.1016/j.xpro.2020.100101
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author Li, Anqi
Qin, Yuan
Gao, Meng
Jiang, Wenting
Liu, Bilin
Tian, Xiangang
Gong, Guohua
author_facet Li, Anqi
Qin, Yuan
Gao, Meng
Jiang, Wenting
Liu, Bilin
Tian, Xiangang
Gong, Guohua
author_sort Li, Anqi
collection PubMed
description We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function in situ. The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protocol, please refer to Gong et al. (2014) and Gong et al. (2015).
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spelling pubmed-75800952020-10-26 Protocol for Imaging of Mitoflashes in Live Cardiomyocytes Li, Anqi Qin, Yuan Gao, Meng Jiang, Wenting Liu, Bilin Tian, Xiangang Gong, Guohua STAR Protoc Protocol We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function in situ. The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protocol, please refer to Gong et al. (2014) and Gong et al. (2015). Elsevier 2020-09-03 /pmc/articles/PMC7580095/ /pubmed/33111128 http://dx.doi.org/10.1016/j.xpro.2020.100101 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Anqi
Qin, Yuan
Gao, Meng
Jiang, Wenting
Liu, Bilin
Tian, Xiangang
Gong, Guohua
Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title_full Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title_fullStr Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title_full_unstemmed Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title_short Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
title_sort protocol for imaging of mitoflashes in live cardiomyocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580095/
https://www.ncbi.nlm.nih.gov/pubmed/33111128
http://dx.doi.org/10.1016/j.xpro.2020.100101
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