Protocol for Imaging of Mitoflashes in Live Cardiomyocytes

We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration fu...

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Detalles Bibliográficos
Autores principales: Li, Anqi, Qin, Yuan, Gao, Meng, Jiang, Wenting, Liu, Bilin, Tian, Xiangang, Gong, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580095/
https://www.ncbi.nlm.nih.gov/pubmed/33111128
http://dx.doi.org/10.1016/j.xpro.2020.100101

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580095/
https://www.ncbi.nlm.nih.gov/pubmed/33111128
http://dx.doi.org/10.1016/j.xpro.2020.100101

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