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Protocol for Immuno-Enrichment of FLAG-Tagged Protein Complexes

This protocol describes immunoprecipitation of proteins associated with FLAG-tagged recombinant proteins followed by mass spectrometry-based proteomics to identify the associated interactome components. FLAG epitope was chosen, because existing high-affinity monoclonal antibodies allow for sensitive...

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Detalles Bibliográficos
Autores principales:	Valdez-Sinon, Arielle Nicole, Gokhale, Avanti, Faundez, Victor, Bassell, Gary Jonathan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2020
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580096/ https://www.ncbi.nlm.nih.gov/pubmed/33111116 http://dx.doi.org/10.1016/j.xpro.2020.100083

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