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Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the anal...

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Detalles Bibliográficos
Autores principales: Liu, Zhaoyuan, Gu, Yaqi, Shin, Amanda, Zhang, Shuangyan, Ginhoux, Florent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580097/
https://www.ncbi.nlm.nih.gov/pubmed/33111080
http://dx.doi.org/10.1016/j.xpro.2020.100029
Descripción
Sumario:Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).