Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry

Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the anal...

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Detalles Bibliográficos
Autores principales: Liu, Zhaoyuan, Gu, Yaqi, Shin, Amanda, Zhang, Shuangyan, Ginhoux, Florent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580097/
https://www.ncbi.nlm.nih.gov/pubmed/33111080
http://dx.doi.org/10.1016/j.xpro.2020.100029
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author Liu, Zhaoyuan
Gu, Yaqi
Shin, Amanda
Zhang, Shuangyan
Ginhoux, Florent
author_facet Liu, Zhaoyuan
Gu, Yaqi
Shin, Amanda
Zhang, Shuangyan
Ginhoux, Florent
author_sort Liu, Zhaoyuan
collection PubMed
description Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).
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spelling pubmed-75800972020-10-26 Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry Liu, Zhaoyuan Gu, Yaqi Shin, Amanda Zhang, Shuangyan Ginhoux, Florent STAR Protoc Protocol Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7580097/ /pubmed/33111080 http://dx.doi.org/10.1016/j.xpro.2020.100029 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Zhaoyuan
Gu, Yaqi
Shin, Amanda
Zhang, Shuangyan
Ginhoux, Florent
Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_full Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_fullStr Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_full_unstemmed Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_short Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
title_sort analysis of myeloid cells in mouse tissues with flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580097/
https://www.ncbi.nlm.nih.gov/pubmed/33111080
http://dx.doi.org/10.1016/j.xpro.2020.100029
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