Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine

The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tissues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high...

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Detalles Bibliográficos
Autores principales: Dhondt, Bert, Lumen, Nicolaas, De Wever, Olivier, Hendrix, An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580105/
https://www.ncbi.nlm.nih.gov/pubmed/33111109
http://dx.doi.org/10.1016/j.xpro.2020.100073
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author Dhondt, Bert
Lumen, Nicolaas
De Wever, Olivier
Hendrix, An
author_facet Dhondt, Bert
Lumen, Nicolaas
De Wever, Olivier
Hendrix, An
author_sort Dhondt, Bert
collection PubMed
description The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tissues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high specificity and repeatability. The implementation of integrative omics allows the study of the molecular complexity of highly purified urinary EV, supporting the identification of EV-specific functions and biomarkers. For complete details on the use and execution of this protocol, please refer to Dhondt et al. (2020).
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spelling pubmed-75801052020-10-26 Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine Dhondt, Bert Lumen, Nicolaas De Wever, Olivier Hendrix, An STAR Protoc Protocol The multidimensional cargo of extracellular vesicles (EV) released in urine is a reflection of the pathophysiological processes occurring within their cells and tissues of origin in the urogenital system. Here, we describe a step-by-step protocol for density-based separation of urinary EV with high specificity and repeatability. The implementation of integrative omics allows the study of the molecular complexity of highly purified urinary EV, supporting the identification of EV-specific functions and biomarkers. For complete details on the use and execution of this protocol, please refer to Dhondt et al. (2020). Elsevier 2020-07-27 /pmc/articles/PMC7580105/ /pubmed/33111109 http://dx.doi.org/10.1016/j.xpro.2020.100073 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Dhondt, Bert
Lumen, Nicolaas
De Wever, Olivier
Hendrix, An
Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title_full Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title_fullStr Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title_full_unstemmed Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title_short Preparation of Multi-omics Grade Extracellular Vesicles by Density-Based Fractionation of Urine
title_sort preparation of multi-omics grade extracellular vesicles by density-based fractionation of urine
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580105/
https://www.ncbi.nlm.nih.gov/pubmed/33111109
http://dx.doi.org/10.1016/j.xpro.2020.100073
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