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Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein

Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular...

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Detalles Bibliográficos
Autores principales: Kojima, Hiroyuki, Itoh, Toshimasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580106/
https://www.ncbi.nlm.nih.gov/pubmed/33111084
http://dx.doi.org/10.1016/j.xpro.2020.100036
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author Kojima, Hiroyuki
Itoh, Toshimasa
author_facet Kojima, Hiroyuki
Itoh, Toshimasa
author_sort Kojima, Hiroyuki
collection PubMed
description Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020).
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spelling pubmed-75801062020-10-26 Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein Kojima, Hiroyuki Itoh, Toshimasa STAR Protoc Protocol Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). Elsevier 2020-06-03 /pmc/articles/PMC7580106/ /pubmed/33111084 http://dx.doi.org/10.1016/j.xpro.2020.100036 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Kojima, Hiroyuki
Itoh, Toshimasa
Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title_full Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title_fullStr Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title_full_unstemmed Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title_short Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
title_sort preparation and use of turn-on fluorescent probe for detection and live cell imaging of vitamin d receptor as a target protein
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580106/
https://www.ncbi.nlm.nih.gov/pubmed/33111084
http://dx.doi.org/10.1016/j.xpro.2020.100036
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