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Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein
Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580106/ https://www.ncbi.nlm.nih.gov/pubmed/33111084 http://dx.doi.org/10.1016/j.xpro.2020.100036 |
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author | Kojima, Hiroyuki Itoh, Toshimasa |
author_facet | Kojima, Hiroyuki Itoh, Toshimasa |
author_sort | Kojima, Hiroyuki |
collection | PubMed |
description | Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). |
format | Online Article Text |
id | pubmed-7580106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75801062020-10-26 Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein Kojima, Hiroyuki Itoh, Toshimasa STAR Protoc Protocol Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). Elsevier 2020-06-03 /pmc/articles/PMC7580106/ /pubmed/33111084 http://dx.doi.org/10.1016/j.xpro.2020.100036 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Kojima, Hiroyuki Itoh, Toshimasa Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title_full | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title_fullStr | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title_full_unstemmed | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title_short | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein |
title_sort | preparation and use of turn-on fluorescent probe for detection and live cell imaging of vitamin d receptor as a target protein |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580106/ https://www.ncbi.nlm.nih.gov/pubmed/33111084 http://dx.doi.org/10.1016/j.xpro.2020.100036 |
work_keys_str_mv | AT kojimahiroyuki preparationanduseofturnonfluorescentprobefordetectionandlivecellimagingofvitamindreceptorasatargetprotein AT itohtoshimasa preparationanduseofturnonfluorescentprobefordetectionandlivecellimagingofvitamindreceptorasatargetprotein |