Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes

Analysis of mitochondrial respiration function represented by the oxygen consumption rate is necessary for assessing mitochondrial respiration function. This protocol describes steps to evaluate the respiration function of mitochondria in situ in saponin-permeabilized cardiomyocytes. In permeabilize...

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Detalles Bibliográficos
Autores principales: Gao, Meng, Li, Anqi, Qin, Yuan, Liu, Bilin, Gong, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580108/
https://www.ncbi.nlm.nih.gov/pubmed/33111108
http://dx.doi.org/10.1016/j.xpro.2020.100072
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author Gao, Meng
Li, Anqi
Qin, Yuan
Liu, Bilin
Gong, Guohua
author_facet Gao, Meng
Li, Anqi
Qin, Yuan
Liu, Bilin
Gong, Guohua
author_sort Gao, Meng
collection PubMed
description Analysis of mitochondrial respiration function represented by the oxygen consumption rate is necessary for assessing mitochondrial respiration function. This protocol describes steps to evaluate the respiration function of mitochondria in situ in saponin-permeabilized cardiomyocytes. In permeabilized cells, mitochondria are in a relatively integrated cellular system, and mitochondrial respiration is more physiologically relevant than isolated mitochondria. For complete details on the use and execution of this protocol, please refer to Gong et al. (2015a) and Gong et al. (2015b).
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spelling pubmed-75801082020-10-26 Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes Gao, Meng Li, Anqi Qin, Yuan Liu, Bilin Gong, Guohua STAR Protoc Protocol Analysis of mitochondrial respiration function represented by the oxygen consumption rate is necessary for assessing mitochondrial respiration function. This protocol describes steps to evaluate the respiration function of mitochondria in situ in saponin-permeabilized cardiomyocytes. In permeabilized cells, mitochondria are in a relatively integrated cellular system, and mitochondrial respiration is more physiologically relevant than isolated mitochondria. For complete details on the use and execution of this protocol, please refer to Gong et al. (2015a) and Gong et al. (2015b). Elsevier 2020-07-25 /pmc/articles/PMC7580108/ /pubmed/33111108 http://dx.doi.org/10.1016/j.xpro.2020.100072 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gao, Meng
Li, Anqi
Qin, Yuan
Liu, Bilin
Gong, Guohua
Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title_full Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title_fullStr Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title_full_unstemmed Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title_short Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes
title_sort protocol for measurement of oxygen consumption rate in situ in permeabilized cardiomyocytes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7580108/
https://www.ncbi.nlm.nih.gov/pubmed/33111108
http://dx.doi.org/10.1016/j.xpro.2020.100072
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